Invention Grant
US06426042B1 Methods and kits for improving retroviral-mediated gene transfer utilizing molecules, or mixture thereof, containing retroviral binding domains and target cell binding domains
失效
使用含有逆转录病毒结合结构域和靶细胞结合结构域的分子或其混合物来改进逆转录病毒介导的基因转移的方法和试剂盒
- Patent Title: Methods and kits for improving retroviral-mediated gene transfer utilizing molecules, or mixture thereof, containing retroviral binding domains and target cell binding domains
- Patent Title (中): 使用含有逆转录病毒结合结构域和靶细胞结合结构域的分子或其混合物来改进逆转录病毒介导的基因转移的方法和试剂盒
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Application No.: US09366009Application Date: 1999-08-02
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Publication No.: US06426042B1Publication Date: 2002-07-30
- Inventor: Kiyozo Asada , Takashi Uemori , Takashi Ueno , Nobuto Koyama , Kimikazu Hashino , Ikunoshin Kato
- Applicant: Kiyozo Asada , Takashi Uemori , Takashi Ueno , Nobuto Koyama , Kimikazu Hashino , Ikunoshin Kato
- Priority: JP7-294382 19951113; JP8-051847 19960308
- Main IPC: G01N3122
- IPC: G01N3122
Abstract:
The present invention provides a kit to carry out retrovirus-mediated gene transfer into target cells. The kit contains a functional material bearing a retrovirus binding domain, another functional material bearing a target cell binding domain, an artificial substrate for incubating the retrovirus contacted with the target cells, and a target cell growth factor for pre-stimulating target cells to spur them along the cell cycle. The kit of the invention may further comprise a recombinant retroviral vector, necessary buffers, and the like.
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