公开(公告)号：US09447388B2

公开(公告)日：2016-09-20

申请号：US14232174

申请日：2012-07-12

申请人： Yoshizumi Ishino ,  Takeshi Yamagami ,  Hiroaki Matsukawa ,  Takashi Uemori ,  Takehiro Sagara

发明人： Yoshizumi Ishino ,  Takeshi Yamagami ,  Hiroaki Matsukawa ,  Takashi Uemori ,  Takehiro Sagara

IPC分类号： C12N9/12 ,  C12N1/00 ,  C12N1/20 ,  C12N15/63 ,  C12P19/34

CPC分类号： C12N9/1252 ,  C12N1/00 ,  C12N1/20 ,  C12N15/63 ,  C12P19/34 ,  C12Y207/07007 ,  Y02P20/52

摘要： Provided are various novel DNA polymerases. Provided is a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 8 by inserting nine amino acids “-A737-A738-A739-A740-A741-A742-A743-A744-A745-” between the amino acid residue at position 736 and the amino acid residue at position 737,wherein: A737 is an amino acid residue having a non-polar aliphatic side chain; A738 is an amino acid residue having a non-polar aliphatic side chain; A739 is an amino acid residue having a positively charged side chain; A740 is an amino acid residue having a positively charged side chain; A741 is an amino acid residue having a non-polar aliphatic side chain; A742 is an amino acid residue having a non-polar aliphatic side chain; A743 is any given amino acid residue; A744 is an amino acid residue having a positively charged side chain; and A745 is an amino acid residue having a non-polar aliphatic side chain).

公开(公告)号：US20130065281A1

公开(公告)日：2013-03-14

申请号：US13697665

申请日：2011-05-09

申请人： Yuko Nakabayashi ,  Takashi Uemori ,  Hiroyuki Mukai ,  Kiyozo Asada

发明人： Yuko Nakabayashi ,  Takashi Uemori ,  Hiroyuki Mukai ,  Kiyozo Asada

IPC分类号： C12P19/34 ,  C12N9/12

CPC分类号： C12N15/1096 ,  C12P19/34

摘要： A method for synthesizing cDNA characterized by preparing a reaction solution that does not allow an endodeoxyribonuclease to show its activity, without thermal deactivation of the endodeoxyribonuclease or removal of the endodeoxyribonuclease, and carrying out a reverse transcription reaction, wherein the reaction solution contains a treated sample and a reverse transcriptase, the treated sample being formed by treating a sample comprising RNA and DNA with the endodeoxyribonuclease to degrade DNA in the sample. The method and the kit for synthesizing cDNA of the present invention are widely useful in genetic engineering fields.

摘要翻译： 一种合成cDNA的方法，其特征在于制备不允许内脱氧核糖核酸酶显示其活性的反应溶液，不脱氧内脱氧核糖核酸酶或去除内切脱氧核糖核酸酶，并进行逆转录反应，其中反应溶液含有处理样品 和逆转录酶，通过用内切核糖核酸酶处理包含RNA和DNA的样品来降解样品中的DNA来形成经处理的样品。 用于合成本发明的cDNA的方法和试剂盒可广泛用于遗传工程领域。

公开(公告)号：US08344105B2

公开(公告)日：2013-01-01

申请号：US13239981

申请日：2011-09-22

申请人： Yoshimi Sato ,  Kazue Nishiwaki ,  Nana Shimada ,  Shigekazu Hokazono ,  Takashi Uemori ,  Hiroyuki Mukai ,  Ikunoshin Kato

发明人： Yoshimi Sato ,  Kazue Nishiwaki ,  Nana Shimada ,  Shigekazu Hokazono ,  Takashi Uemori ,  Hiroyuki Mukai ,  Ikunoshin Kato

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07K1/00

CPC分类号： C12Q1/686 ,  C12N9/1252 ,  C12Q2521/101

摘要： A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.

摘要翻译： 具有高保真DNA聚合酶活性的多肽，因此可用作遗传工程试剂; 编码该多肽的基因; 一种产生该多肽的方法; 以及通过使用多肽扩增核酸的方法。

公开(公告)号：US07790849B2

公开(公告)日：2010-09-07

申请号：US12241581

申请日：2008-09-30

申请人： Kiyozo Asada ,  Takashi Uemori ,  Takashi Ueno ,  Nobuto Koyama ,  Kimikazu Hashino ,  Ikunoshin Kato

发明人： Kiyozo Asada ,  Takashi Uemori ,  Takashi Ueno ,  Nobuto Koyama ,  Kimikazu Hashino ,  Ikunoshin Kato

IPC分类号： C07K14/00 ,  C07H21/04

CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/505 ,  C07K14/78 ,  C07K2319/00 ,  C12N9/1088 ,  C12N2740/13043 ,  C12N2740/13045

摘要： An isolated polypeptide includes the amino acid sequence shown in SEQ. ID No. 13 and an isolated nucleic acid encoding the polypeptide, such as an isolated nucleic acid including the nucleic acid sequence shown in SEQ. ID No. 17. The isolated polypeptide includes two heparin binding polypeptides connected in tandem and the isolated nucleic acid encodes these. The polypeptide and nucleic acid sequences can improve retroviral vector mediated gene transfer efficiency into target cells.

摘要翻译： 分离的多肽包括SEQ ID NO：1所示的氨基酸序列。 ID号13和编码该多肽的分离的核酸，例如分离的核酸，包括SEQ ID NO：1所示的核酸序列。 分离的多肽包括串联连接的两个肝素结合多肽，分离的核酸编码这些。 多肽和核酸序列可以改进逆转录病毒载体介导的靶细胞的基因转移效率。

公开(公告)号：US07572617B2

公开(公告)日：2009-08-11

申请号：US10526073

申请日：2003-08-26

申请人： Shigekazu Hokazono ,  Takashi Uemori ,  Tetsuki Tanaka ,  Ikunoshin Kato

发明人： Shigekazu Hokazono ,  Takashi Uemori ,  Tetsuki Tanaka ,  Ikunoshin Kato

IPC分类号： C07K14/00 ,  C12N9/16 ,  C12Q1/14 ,  C12N15/00 ,  C12N5/10 ,  C12N1/21 ,  C12P21/00 ,  C07H21/00

CPC分类号： C12N9/22

摘要： An isolated polypeptide having a thermostable ribonuclease H activity from Archaeoglobus profundus is highly useful in genetic engineering, as well as a gene encoding this polypeptide and a genetic engineering process for producing the polypeptide.

摘要翻译： 具有来自古细丝虫的热稳定核糖核酸酶H活性的分离的多肽在遗传工程中是非常有用的，以及编码该多肽的基因和用于产生多肽的基因工程方法。

公开(公告)号：US20060030046A1

公开(公告)日：2006-02-09

申请号：US11181091

申请日：2005-07-14

申请人： Kiyozo Asada ,  Takashi Uemori ,  Takashi Ueno ,  Nobuto Koyama ,  Kimikazu Hashino ,  Ikunoshin Kato

发明人： Kiyozo Asada ,  Takashi Uemori ,  Takashi Ueno ,  Nobuto Koyama ,  Kimikazu Hashino ,  Ikunoshin Kato

IPC分类号： C12N15/867 ,  C07K14/50

CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/505 ,  C07K14/78 ,  C07K2319/00 ,  C12N9/1088 ,  C12N2740/13043 ,  C12N2740/13045

摘要： A polypeptide represented by SEQ. ID No. 13, a polypeptide represented by SEQ. ID No. 30 or functional equivalents thereof and a polypeptide represented by SEQ. ID No. 17.

摘要翻译： SEQ ID NO： SEQ ID NO：13，SEQ ID NO： SEQ ID No 30或其功能等同物和SEQ ID NO： ID号17。

公开(公告)号：US5189144A

公开(公告)日：1993-02-23

申请号：US852343

申请日：1992-03-17

申请人： Kiyozo Asada ,  Takashi Uemori ,  Masahiro Endo ,  Fusao Kimizuka ,  Ikunoshin Kato

发明人： Kiyozo Asada ,  Takashi Uemori ,  Masahiro Endo ,  Fusao Kimizuka ,  Ikunoshin Kato

IPC分类号： C07K7/08 ,  C07K14/81

CPC分类号： C07K7/08 ,  C07K14/8139

摘要： Polypeptides are provided which have a calpain inhibitory activity. The polypeptides have an activity like that of natural human calpastatin but are significantly shorter in length than natural human calpastatin. The polypeptides are suited for synthetic manufacture.

摘要翻译： 提供具有钙蛋白酶抑制活性的多肽。 多肽具有类似于天然人钙蛋白酶抑制剂的活性，但其长度明显短于天然人钙蛋白酶抑制剂。 该多肽适用于合成制造。

公开(公告)号：US09458498B2

公开(公告)日：2016-10-04

申请号：US13980970

申请日：2012-01-20

申请人： Junko Yamamoto ,  Keiko Kubo ,  Takashi Uemori ,  Hiroyuki Mukai ,  Kiyozo Asada

发明人： Junko Yamamoto ,  Keiko Kubo ,  Takashi Uemori ,  Hiroyuki Mukai ,  Kiyozo Asada

IPC分类号： C12Q1/68 ,  C12N15/01

CPC分类号： C12N15/01 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q1/689 ,  C12Q2523/101 ,  C12Q2525/117

摘要： The present invention pertains to: a method for modifying a nucleic acid contained in a sample, the method including a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, the method including the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.

摘要翻译： 本发明涉及：用于修饰样品中所含的核酸的方法，所述方法包括在酸性多糖和/或核苷酸的存在下使样品与核酸改性剂接触的步骤; 以及选择性检测来自样品中含有的活细胞的核酸的方法，所述方法包括以下步骤：（a）根据包含在所述样品中的核酸的方法修饰样品中的核酸的步骤 样品，其包括在酸性多糖和/或核苷酸的存在下使样品与核酸改性剂接触的步骤; 和（b）在步骤（a）之后从样品中选择性地检测未修饰的核酸的步骤。 本发明还涉及用于这些方法的试剂盒和组合物。

公开(公告)号：US09200314B2

公开(公告)日：2015-12-01

申请号：US13186941

申请日：2011-07-20

申请人： Satoshi Yanagi ,  Eiji Kobayashi ,  Takashi Uemori ,  Hiroyuki Mukai

发明人： Satoshi Yanagi ,  Eiji Kobayashi ,  Takashi Uemori ,  Hiroyuki Mukai

IPC分类号： C12P19/34 ,  C12Q1/68 ,  C12N15/10 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6806 ,  C12N15/1096 ,  C12Q2525/173 ,  C12Q2525/207

摘要： A method for accurately and easily detecting a synthetic siRNA, for example, a siRNA in which the 3′ end is DNA, and a kit used for the method are provided. The present invention relates to a method for detecting a siRNA in which the 3′ end is DNA, comprising: (a) adding polydeoxyadenosine to the 3′ DNA end of at least one strand of the siRNA to be detected to produce a polydeoxyadenosine-added RNA; (b) annealing a polydeoxythymidine primer having a tag sequence at its 5′ side to the polydeoxyadenosine-added RNA and synthesizing DNA from the primer by a reverse transcription; and (c) detecting the DNA synthesized in (b).

摘要翻译： 提供了准确且容易地检测合成siRNA的方法，例如其中3'末端为DNA的siRNA和用于该方法的试剂盒。 本发明涉及一种用于检测其中3'末端为DNA的siRNA的方法，包括：（a）向被检测的siRNA的至少一条链的3'DNA末端添加多脱氧腺苷以产生加入的多脱氧腺苷 RNA; （b）将其5'侧具有标签序列的多脱氧胸苷引物退火至加入多脱氧腺苷的RNA并通过逆转录合成来自引物的DNA; 和（c）检测（b）中合成的DNA。

公开(公告)号：US20140322793A1

公开(公告)日：2014-10-30

申请号：US14232174

申请日：2012-07-12

申请人： Yoshizumi Ishino ,  Takeshi Yamagami ,  Hiroaki Matsukawa ,  Takashi Uemori ,  Takehiro Sagara

发明人： Yoshizumi Ishino ,  Takeshi Yamagami ,  Hiroaki Matsukawa ,  Takashi Uemori ,  Takehiro Sagara

IPC分类号： C12N9/12

CPC分类号： C12N9/1252 ,  C12N1/00 ,  C12N1/20 ,  C12N15/63 ,  C12P19/34 ,  C12Y207/07007 ,  Y02P20/52

摘要： Provided are various novel DNA polymerases. Provided is a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 8 by inserting nine amino acids “-A737-A738-A739-A740-A741-A742-A743-A744-A745-” between the amino acid residue at position 736 and the amino acid residue at position 737,wherein: A737 is an amino acid residue having a non-polar aliphatic side chain; A738 is an amino acid residue having a non-polar aliphatic side chain; A739 is an amino acid residue having a positively charged side chain; A740 is an amino acid residue having a positively charged side chain; A741 is an amino acid residue having a non-polar aliphatic side chain; A742 is an amino acid residue having a non-polar aliphatic side chain; A743 is any given amino acid residue; A744 is an amino acid residue having a positively charged side chain; and A745 is an amino acid residue having a non-polar aliphatic side chain).

摘要翻译： 提供各种新型DNA聚合酶。 本发明提供一种DNA聚合酶，其包含在SEQ ID NO：8的氨基酸序列之间插入9个氨基酸“-A737-A738-A739-A740-A741-A742-A743-A744-A745-”的氨基酸序列 位置736处的氨基酸残基和737位的氨基酸残基，其中：A737是具有非极性脂肪族侧链的氨基酸残基; A738是具有非极性脂肪族侧链的氨基酸残基; A739是具有带正电侧链的氨基酸残基; A740是具有带正电荷侧链的氨基酸残基; A741是具有非极性脂肪族侧链的氨基酸残基; A742是具有非极性脂肪族侧链的氨基酸残基; A743是任何给定的氨基酸残基; A744是具有带正电侧链的氨基酸残基; A745是具有非极性脂肪族侧链的氨基酸残基）。