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发明授权
US06444429B1 Gene coding for DNA ligase of hyperthermophilic bacteria Aquifex pyrophilus and protein expressed therefrom 失效
基因编码嗜热菌嗜酸菌的DNA连接酶Aquifex pyrophilus和由其表达的蛋白质

  • 专利标题： Gene coding for DNA ligase of hyperthermophilic bacteria Aquifex pyrophilus and protein expressed therefrom
  • 专利标题（中）： 基因编码嗜热菌嗜酸菌的DNA连接酶Aquifex pyrophilus和由其表达的蛋白质
  • 申请号： US09708426
    申请日： 2000-11-09
  • 公开(公告)号： US06444429B1
    公开(公告)日： 2002-09-03
  • 发明人: Ye-Sun Han , Yeon-Gyu Yu , Jae-Hwan Lim
  • 申请人： Ye-Sun Han , Yeon-Gyu Yu , Jae-Hwan Lim
  • 优先权： KR1999-49591 19991110
  • 主分类号： C12Q168
  • IPC分类号： C12Q168
Gene coding for DNA ligase of hyperthermophilic bacteria Aquifex pyrophilus and protein expressed therefrom
摘要：
The present invention relates to a gene coding for a thermostable DNA ligase from Aquifex pyrophilus, a hyperthermophilic Bacterium, a protein having an amino acid sequence expressed therefrom and a probe used for detecting said ligase DNA. More particularly, the present invention is directed to a gene having a base sequence of SEQ. ID. NO: 3 and a thermostable DNA ligase having an amino acid sequence expressed therefrom (SEQ. ID. NO: 4). Since Aquifex pyrophilus DNA ligase of the present invention has 75% of the ligation activity after heating at 95 ° C. for 1 hour, the ligase has higher stability than DNA ligases from the preexisting bacteria under high temperature and thus it can be advantageously used in genetic diseases assay of medical field, etc.
摘要（中）：

本发明涉及编码来自Aquifex pyrophilus的热稳定DNA连接酶的基因，超嗜热细菌，具有由其表达的氨基酸序列的蛋白质和用于检测所述连接酶DNA的探针。 更具体地，本发明涉及具有SEQ ID NO：1的碱基序列的基因。 ID。 NO.3和具有由其表达的氨基酸序列的热稳定DNA连接酶（SEQ ID NO：4）。由于本发明的Aquifex pyrophilus DNA连接酶在95℃加热后具有75％的连接活性，为1 小时，连接酶在高温下比来自预先存在的细菌的DNA连接酶具有更高的稳定性，因此可有利地用于医疗领域的遗传疾病测定等。

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