公开(公告)号：US06444429B1

公开(公告)日：2002-09-03

申请号：US09708426

申请日：2000-11-09

申请人： Ye-Sun Han ,  Yeon-Gyu Yu ,  Jae-Hwan Lim

发明人： Ye-Sun Han ,  Yeon-Gyu Yu ,  Jae-Hwan Lim

IPC分类号： C12Q168

CPC分类号： C12N9/93 ,  C12Q1/6876 ,  C12Q2521/501

摘要： The present invention relates to a gene coding for a thermostable DNA ligase from Aquifex pyrophilus, a hyperthermophilic Bacterium, a protein having an amino acid sequence expressed therefrom and a probe used for detecting said ligase DNA. More particularly, the present invention is directed to a gene having a base sequence of SEQ. ID. NO: 3 and a thermostable DNA ligase having an amino acid sequence expressed therefrom (SEQ. ID. NO: 4). Since Aquifex pyrophilus DNA ligase of the present invention has 75% of the ligation activity after heating at 95 ° C. for 1 hour, the ligase has higher stability than DNA ligases from the preexisting bacteria under high temperature and thus it can be advantageously used in genetic diseases assay of medical field, etc.

摘要翻译： 本发明涉及编码来自Aquifex pyrophilus的热稳定DNA连接酶的基因，超嗜热细菌，具有由其表达的氨基酸序列的蛋白质和用于检测所述连接酶DNA的探针。 更具体地，本发明涉及具有SEQ ID NO：1的碱基序列的基因。 ID。 NO.3和具有由其表达的氨基酸序列的热稳定DNA连接酶（SEQ ID NO：4）。由于本发明的Aquifex pyrophilus DNA连接酶在95℃加热后具有75％的连接活性，为1 小时，连接酶在高温下比来自预先存在的细菌的DNA连接酶具有更高的稳定性，因此可有利地用于医疗领域的遗传疾病测定等。