发明授权
US06472180B1 HCV NS3 protein fragments having helicase activity and improved solubility
有权
具有解旋酶活性和改善溶解性的HCV NS3蛋白片段
- 专利标题： HCV NS3 protein fragments having helicase activity and improved solubility
- 专利标题（中）： 具有解旋酶活性和改善溶解性的HCV NS3蛋白片段
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申请号： US09483799申请日： 2000-01-15
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公开(公告)号： US06472180B1公开(公告)日： 2002-10-29
- 发明人: Michael Houghton , Qui-Lim Choo , Jang Han , Joonho Choe
- 申请人： Michael Houghton , Qui-Lim Choo , Jang Han , Joonho Choe
- 主分类号： C12N1562
- IPC分类号： C12N1562
摘要：
The Hepatitis C Virus (HCV) NS3 protein contains amino acid motifs of a serine proteinase, a nucleotide triphosphatase (NTPase), and an RNA helicase. A carboxy fragment of the HCV NS3 protein was purified and possessed RNA helicase activity. Detections from the amino terminus resulted in the protein becoming soluble. Deletions from the carboxy terminus do not result in a loss of helicase activity until at least 50 amino acids are deleted. The helicase activity requires ATP and divalent cations such as Mg2+ and Mn2+. The helicase activity was blocked by monoclonal antibody specific to the HCV NS3 protein.
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