公开(公告)号：US5989905A

公开(公告)日：1999-11-23

申请号：US833678

申请日：1997-04-08

申请人： Michael Houghton ,  Qui-Lim Choo ,  Jang Han ,  Joonho Choe

发明人： Michael Houghton ,  Qui-Lim Choo ,  Jang Han ,  Joonho Choe

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61P31/20 ,  A61P43/00 ,  C07K14/00 ,  C07K14/18 ,  C07K19/00 ,  C12N1/21 ,  C12N9/00 ,  C12N9/02 ,  C12N9/22 ,  C12N9/50 ,  C12N9/90 ,  C12N15/00 ,  C12N15/57 ,  C12R1/19 ,  C12R1/92 ,  C07H21/04

CPC分类号： C07K14/005 ,  C07K14/00 ,  C12N9/0089 ,  C12N9/506 ,  C12N9/90 ,  A61K38/00 ,  C07K2319/00 ,  C12N2770/24222

摘要： The Hepatitis C Virus (HCV) NS3 protein contains amino acid motifs of a serine proteinase, a nucleotide triphosphatase (NTPase), and an RNA helicase. A carboxy fragment of the HCV NS3 protein was purified and possessed RNA helicase activity. Detections from the amino terminus resulted in the protein becoming soluble. Deletions from the carboxy terminus do not result in a loss of helicase activity until at least 50 amino acids are deleted. The helicase activity requires ATP and divalent cations such as Mg.sup.2+ and Mn.sup.2+. The helicase activity was blocked by monoclonal antibody specific to the HCV NS3 protein.

公开(公告)号：US06472180B1

公开(公告)日：2002-10-29

申请号：US09483799

申请日：2000-01-15

申请人： Michael Houghton ,  Qui-Lim Choo ,  Jang Han ,  Joonho Choe

发明人： Michael Houghton ,  Qui-Lim Choo ,  Jang Han ,  Joonho Choe

IPC分类号： C12N1562

CPC分类号： C07K14/005 ,  A61K38/00 ,  C07K14/00 ,  C07K2319/00 ,  C12N9/0089 ,  C12N9/506 ,  C12N9/90 ,  C12N2770/24222

摘要： The Hepatitis C Virus (HCV) NS3 protein contains amino acid motifs of a serine proteinase, a nucleotide triphosphatase (NTPase), and an RNA helicase. A carboxy fragment of the HCV NS3 protein was purified and possessed RNA helicase activity. Detections from the amino terminus resulted in the protein becoming soluble. Deletions from the carboxy terminus do not result in a loss of helicase activity until at least 50 amino acids are deleted. The helicase activity requires ATP and divalent cations such as Mg2+ and Mn2+. The helicase activity was blocked by monoclonal antibody specific to the HCV NS3 protein.

摘要翻译： 丙型肝炎病毒（HCV）NS3蛋白含有丝氨酸蛋白酶，核苷酸三磷酸酶（NTPase）和RNA解旋酶的氨基酸基序。 HCV NS3蛋白的羧基片段被纯化并具有RNA解旋酶活性。 氨基末端的检测导致蛋白质变得可溶。 从羧基末端的缺失不会导致直到至少50个氨基酸被缺失的解旋酶活性丧失。 解旋酶活性需要ATP和二价阳离子如Mg2 +和Mn2 +。 解旋酶活性被HCV NS3蛋白特异性的单克隆抗体阻断。

公开(公告)号：US07033805B2

公开(公告)日：2006-04-25

申请号：US10232643

申请日：2002-11-25

申请人： Michael Houghton ,  Qui-Lim Choo ,  Jang Han ,  Joonho Choe

发明人： Michael Houghton ,  Qui-Lim Choo ,  Jang Han ,  Joonho Choe

IPC分类号： C12N9/00 ,  C12N9/10 ,  C07H21/04

CPC分类号： C07K14/005 ,  A61K38/00 ,  C07K14/00 ,  C07K2319/00 ,  C12N9/0089 ,  C12N9/506 ,  C12N9/90 ,  C12N2770/24222

摘要： The Hepatitis C Virus (HCV) NS3 protein contains amino acid motifs of a serine proteinase, a nucleotide triphosphatase (NTPase), and an RNA helicase. A carboxy fragment of the HCV NS3 protein was purified and possessed RNA helicase activity. Detections from the amino terminus resulted in the protein becoming soluble. Deletions from the carboxy terminus do not result in a loss of helicase activity until at least 50 amino acids are deleted. The helicase activity requires ATP and divalent cations such as Mg2+ and Mn2+. The helicase activity was blocked by monoclonal antibody specific to the HCV NS3 protein.

摘要翻译： 丙型肝炎病毒（HCV）NS3蛋白含有丝氨酸蛋白酶，核苷酸三磷酸酶（NTPase）和RNA解旋酶的氨基酸基序。 HCV NS3蛋白的羧基片段被纯化并具有RNA解旋酶活性。 氨基末端的检测导致蛋白质变得可溶。 从羧基末端的缺失不会导致直到至少50个氨基酸被缺失的解旋酶活性丧失。 解旋酶活性需要ATP和二价阳离子如Mg 2+和Mn 2+。 解旋酶活性被针对HCV NS3蛋白特异性的单克隆抗体阻断。

公开(公告)号：US06194140B1

公开(公告)日：2001-02-27

申请号：US08529169

申请日：1995-09-15

申请人： Michael Houghton ,  Qui-Lim Choo ,  Jang Han ,  Joonho Choe

发明人： Michael Houghton ,  Qui-Lim Choo ,  Jang Han ,  Joonho Choe

IPC分类号： C12Q100

CPC分类号： C07K14/005 ,  A61K38/00 ,  C07K14/00 ,  C07K2319/00 ,  C12N9/0089 ,  C12N9/506 ,  C12N9/90 ,  C12N2770/24222

摘要： The Hepatitis C Virus (HCV) NS3 protein contains amino acid motifs of a serine proteinase, a nucleotide triphosphatase (NTPase), and an RNA helicase. A carboxy fragment of the HCV NS3 protein was purified and possessed RNA helicase activity. Deletions from the amino terminus resulted in the protein becoming soluble. Deletions from the carboxy terminus do not result in a loss of helicase activity until at least 50 amino acids are deleted. The helicase activity requires ATP and divalent cations such as Mg2+ and Mn2+. The helicase activity was blocked by monoclonal antibody specific to the HCV NS3 protein.

摘要翻译： 丙型肝炎病毒（HCV）NS3蛋白含有丝氨酸蛋白酶，核苷酸三磷酸酶（NTPase）和RNA解旋酶的氨基酸基序。 HCV NS3蛋白的羧基片段被纯化并具有RNA解旋酶活性。 从氨基末端的缺失导致蛋白质变得可溶。 从羧基末端的缺失不会导致直到至少50个氨基酸被缺失的解旋酶活性丧失。 解旋酶活性需要ATP和二价阳离子如Mg2 +和Mn2 +。 解旋酶活性被HCV NS3蛋白特异性的单克隆抗体阻断。