发明授权
US06472204B1 Methods for retroviral mediated gene transfer employing molecules, or mixtures thereof, containing retroviral binding domains and target cell binding domains
失效
使用含有逆转录病毒结合结构域和靶细胞结合结构域的分子或其混合物进行逆转录病毒介导的基因转移的方法
- 专利标题： Methods for retroviral mediated gene transfer employing molecules, or mixtures thereof, containing retroviral binding domains and target cell binding domains
- 专利标题（中）： 使用含有逆转录病毒结合结构域和靶细胞结合结构域的分子或其混合物进行逆转录病毒介导的基因转移的方法
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申请号： US08809156申请日： 1997-03-07
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公开(公告)号： US06472204B1公开(公告)日： 2002-10-29
- 发明人: Kiyozo Asada , Takashi Uemori , Takashi Ueno , Nobuto Koyama , Kimakazu Hasino , Ikunoshin Kato
- 申请人： Kiyozo Asada , Takashi Uemori , Takashi Ueno , Nobuto Koyama , Kimakazu Hasino , Ikunoshin Kato
- 优先权： JP7-294382 19951113; JP8-051847 19960308
- 主分类号： C12N1500
- IPC分类号： C12N1500
摘要：
A method is disclosed for increasing the efficiency of retroviral mediated gene transfer into viable target cells, which comprises transducing the target cells by infecting the target cells with a replication defective recombinant retrovirus that infects the target cells in an aqueous medium in the presence of (a) a mixture of an effective amount of a first functional material having a retrovirus binding domain that binds said retrovirus, and an effective amount of a second functional material having a target cell binding domain that binds said target cell, or (b) an effective amount of a bifunctional material having both a retroviral binding domain which does not contain the heparin binding domain derived from human fibronectin, and a target cell binding domain, wherein the bifunctional material has a retrovirus binding domain that binds to said retrovirus and a target cell binding domain that binds to the target cell.