发明授权
- 专利标题： High expression system of proteins
- 专利标题（中）： 蛋白质高表达系统
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申请号： US09517529申请日： 2000-03-02
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公开(公告)号： US06558920B1公开(公告)日： 2003-05-06
- 发明人: Yoji Hata , Hiroki Ishida , Hiromoto Hisada , Eiji Ichikawa , Akitsugu Kawato , Yasuhisa Abe , Koji Suginami , Satoshi Imayasu
- 申请人： Yoji Hata , Hiroki Ishida , Hiromoto Hisada , Eiji Ichikawa , Akitsugu Kawato , Yasuhisa Abe , Koji Suginami , Satoshi Imayasu
- 优先权： JP11-154271 19990601; JP2000-036754 20000215
- 主分类号： C12N934
- IPC分类号： C12N934
摘要：
According to the protein expression system in which a variety of desired useful proteins are highly produced by fusing a coding region of useful protein gene to the downstream of a promoter region of a tyrosinase-encoding gene (melO) of Aspergillus oryzae by a usual method of DNA manipulation, transferring a plasmid containing the resulting novel fusion gene into Aspergillus oryzae and incubating the thus-obtained transformant, various proteins can efficiently be produced at a high purity and in a high yield.
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