发明授权
US06759532B2 Method for obtaining DNA from fish spermatogonium 失效
从鱼类精原细菌获得DNA的方法

  • 专利标题: Method for obtaining DNA from fish spermatogonium
  • 专利标题(中): 从鱼类精原细菌获得DNA的方法
  • 申请号: US10029281
    申请日: 2001-12-28
  • 公开(公告)号: US06759532B2
    公开(公告)日: 2004-07-06
  • 发明人: Han Oh ParkIn Woo Lee
  • 申请人: Han Oh ParkIn Woo Lee
  • 优先权: KR10-1999-0046220 19991023
  • 主分类号: C07H2100
  • IPC分类号: C07H2100
Method for obtaining DNA from fish spermatogonium
摘要:
A method for obtaining DNA from fish spermatogonia comprises (i) disrupting fish spermatogonium to obtain a milky-white colloid, (ii) adding an alkaline solution of pH 8 to pH 12, containing more than 1 mol of salts, such as monovalent salts, to the milky-white colloid, to separate DNA from protamines, and then (iii) adding ethanol solution to the resultant mixture, effecting a precipitation of DNA.
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