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    Method for obtaining DNA from fish spermatogonium
    1.
    发明授权
    Method for obtaining DNA from fish spermatogonium 失效
    从鱼类精原细菌获得DNA的方法

    公开(公告)号：US06759532B2

    公开(公告)日：2004-07-06

    申请号：US10029281

    申请日：2001-12-28

    申请人： Han Oh Park ,  In Woo Lee

    发明人： Han Oh Park ,  In Woo Lee

    IPC分类号： C07H2100

    CPC分类号： C12N15/1003

    摘要： A method for obtaining DNA from fish spermatogonia comprises (i) disrupting fish spermatogonium to obtain a milky-white colloid, (ii) adding an alkaline solution of pH 8 to pH 12, containing more than 1 mol of salts, such as monovalent salts, to the milky-white colloid, to separate DNA from protamines, and then (iii) adding ethanol solution to the resultant mixture, effecting a precipitation of DNA.

    摘要翻译： 从鱼类精原细菌获得DNA的方法包括：（i）破碎鱼类精母细胞以获得乳白色胶体，（ii）将pH8的碱性溶液加入pH12，含有多于1摩尔的盐，如一价盐， 向乳白色胶体中分离DNA，然后（iii）向所得混合物中加入乙醇溶液，进行DNA的沉淀。

    Automatic necleic acid purification apparatus and method for aerosol-protecting
    2.
    发明授权
    Automatic necleic acid purification apparatus and method for aerosol-protecting 有权
    自动碳酸净化装置及气溶胶保护方法

    公开(公告)号：US09273306B2

    公开(公告)日：2016-03-01

    申请号：US13885556

    申请日：2011-11-18

    申请人： Han Oh Park ,  Byung Rae Jeong ,  Kwon Sic Kim

    发明人： Han Oh Park ,  Byung Rae Jeong ,  Kwon Sic Kim

    IPC分类号： C12Q1/68 ,  B01L3/00 ,  C12N15/10

    CPC分类号： C12N15/1013 ,  C12Q1/68

    摘要： Disclosed is an automatic nucleic acid purification apparatus which can prevent pollution due to aerosol generated from a biological sample containing high concentration target nucleic acid when the biological sample containing the high concentration target nucleic acid is mixed with other biological sample containing low concentration target nucleic acid or not containing the target nucleic acid. Further, disclosed is an automatic nucleic acid purification apparatus which can be applied to all kinds of nucleic acid purification equipments for purifying a plurality of biological samples using a magnet rode or a multi-pipette block moving in two or three axial directions, and which can minimize pollution due to the aerosol generated from the biological sample containing high concentration target nucleic acid and also can obtain accurate results.

    摘要翻译： 公开了一种自动核酸纯化装置，当含有高浓度靶核酸的生物样品与含有低浓度靶核酸的其他生物样品混合时，可以防止由含有高浓度靶核酸的生物样品产生的气溶胶引起的污染，或 不含靶核酸。 此外，公开了一种自动核酸纯化装置，其可以应用于使用磁珠或多个移液管块在两个或三个轴向移动的多个生物样品的各种核酸纯化设备，并且其可以 尽可能减少由含有高浓度靶核酸的生物样品产生的气溶胶引起的污染，并可获得准确的结果。

    METHOD OF IDENTIFYING NUCLEIC ACID-CONTAINING OBJECT
    5.
    发明申请
    METHOD OF IDENTIFYING NUCLEIC ACID-CONTAINING OBJECT 审中-公开
    识别含有核酸的物体的方法

    公开(公告)号：US20140087377A1

    公开(公告)日：2014-03-27

    申请号：US14005213

    申请日：2012-03-13

    申请人： Han Oh Park ,  Jung Young Choi ,  Eun Su Han ,  Gu Young Song ,  Won Seok Jang

    发明人： Han Oh Park ,  Jung Young Choi ,  Eun Su Han ,  Gu Young Song ,  Won Seok Jang

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6816 ,  C12Q1/6818 ,  C12Q2521/327 ,  C12Q2525/121 ,  C12Q2563/185

    摘要： The present invention relates to a method of identifying nucleic acid-containing object, more precisely a method of identifying nucleic acid-containing object which comprises the following steps: (1) preparing nucleic acid-containing object having the nucleotide sequence complementary to the nucleotide sequence of RNA-dual probe; (2) reacting the nucleic acid included in the object with the buffer containing the RNA-dual probe conjugated with a reporter and a quencher respectively and RNase; and (3) detecting fluorescence generated from the reporter. The method of identifying an object of the present invention provides labeling sensitivity 100 times as high as that of the conventional method using sequencing or labeling with fluorescent materials, takes advantages of shorter analysis time, facilitates different labeling on a variety of products according to fluorescent materials, and makes possible unlimited product administration by product group and batch in real production process by differentiating the nucleotide sequence of each oligonucleotide.

    摘要翻译： 本发明涉及鉴定含核酸物质的方法，更准确地说，一种鉴定含核酸物质的方法，包括以下步骤：（1）制备具有与核苷酸序列互补的核苷酸序列的含核酸物质 的RNA双探针; （2）将包含在物体中的核酸与分别与报告子和猝灭剂结合的RNA双重探针的缓冲液和RNase分别反应; 和（3）检测从报告物产生的荧光。 识别本发明的目的的方法提供了使用荧光材料进行测序或标记的常规方法的100倍的标签灵敏度，具有较短的分析时间的优点，便于根据荧光材料对各种产品进行不同的标记 并通过区分每个寡核苷酸的核苷酸序列，在实际生产过程中使产品组和批次无限制地进行产品管理。

    Primers for PCR amplification comprising a basic parts within the primer sequences
    9.
    发明授权
    Primers for PCR amplification comprising a basic parts within the primer sequences 有权
    用于PCR扩增的引物，其包含引物序列内的基本部分

    公开(公告)号：US08513399B2

    公开(公告)日：2013-08-20

    申请号：US12681754

    申请日：2008-10-02

    申请人： Hyun Bae Kim ,  Seong Youl Kim ,  Jun Mo Gil ,  Hae Joon Park ,  Han Oh Park

    发明人： Hyun Bae Kim ,  Seong Youl Kim ,  Jun Mo Gil ,  Hae Joon Park ,  Han Oh Park

    IPC分类号： C07H21/04 ,  C07H19/04 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/686 ,  C12Q2525/119

    摘要： The present invention relates to primers for PCR amplification comprising abasic parts within the primer sequences and a method for PCR amplification using the same. More precisely, the present invention relates to primers capable of amplifying different templates and having abasic parts complementary to mutated site or polymorphic site of template DNA and a method for PCR amplification comprising the steps of mixing the composition for PCR amplification comprising the primers with nucleic acid template; and performing PCR with the mixture. The primers for PCR amplification of the present invention contain abasic parts not having specific coding information in their nucleotide sequences, so that they can amplify different templates having mutated sites at the same time.

    摘要翻译： 本发明涉及用于PCR扩增的引物，其包括引物序列内的无碱部分和使用其的PCR扩增方法。 更确切地说，本发明涉及能够扩增不同模板并具有与模板DNA的突变位点或多态位点互补的脱碱基的引物，以及用于PCR扩增的方法，其包括将包含引物的PCR扩增组合物与核酸 模板; 并用混合物进行PCR。 用于本发明的PCR扩增的引物含有在其核苷酸序列中不具有特定编码信息的脱碱基，从而可以同时扩增具有突变位点的不同模板。

    Real-time PCR monitoring apparatus
    10.
    发明授权
    Real-time PCR monitoring apparatus 有权
    实时PCR监测仪

    公开(公告)号：US08427643B2

    公开(公告)日：2013-04-23

    申请号：US12598061

    申请日：2008-06-17

    申请人： Hanee Park ,  Il kyu Choi ,  Han Oh Park

    发明人： Hanee Park ,  Il kyu Choi ,  Han Oh Park

    IPC分类号： G01J4/00

    CPC分类号： G01N21/6445 ,  G01J3/0224 ,  G01J3/4406 ,  G01N21/6452 ,  G01N2021/6478 ,  G01N2021/6484 ,  G01N2201/0631 ,  G01N2201/0636

    摘要： The present invention relates to a real-time PCR monitoring apparatus for real-time monitoring production of reaction product produced during the reaction while performing nucleic acid amplification such as PCR for various kinds of trace samples. Specifically, the present invention relates to an apparatus for real-time monitoring biochemical reaction for efficiently dividing interference between an excitation light and a fluorescence, which includes a polarizer, a polarizing beam splitter, a polarization converter and so on.

    摘要翻译： 本发明涉及一种用于实时监测在反应过程中产生的反应产物的实时PCR监测装置，同时对各种痕量样品进行核酸扩增如PCR。 具体地说，本发明涉及一种用于实时监测生化反应的装置，用于有效地分离激发光和荧光之间的干扰，其包括偏振器，偏振分束器，偏振转换器等。