发明授权
- 专利标题： Modified naphthalene dioxygenases and methods of use
- 专利标题（中）： 改性萘双加氧酶及其使用方法
-
申请号： US09843250申请日： 2001-04-26
-
公开(公告)号： US06794167B2公开(公告)日： 2004-09-21
- 发明人: Rebecca Parales , David Gibson , Sol Resnick , Kyoung Lee
- 申请人： Rebecca Parales , David Gibson , Sol Resnick , Kyoung Lee
- 主分类号： C12N902
- IPC分类号： C12N902
摘要：
The invention provides an NDO or NDO related complex comprising at least one alpha-subunit polypeptide that comprises: 1) a substituted amino acid at the position corresponding to position 352 in NDO, 2) a substituted amino acid at the position corresponding to position 201, 202, 260, 316, 351, 358, 362, or 366 in NDO, or 3) a substituted amino acid at the position corresponding to position 352 in NDO, and a substituted amino acid at the position corresponding to position 201, 202, 260, 316, 351, 358, 362, or 366 in NDO; or a catalytically active fragment thereof. The invention also provides DNA encoding such polypeptides, host cells augmented by such DNA, and methods for using the enzymes or host cells to provide useful and novel synthons. The invention also provides novel compounds prepared with the complexes or methods of the invention.
公开/授权文献
- US20030022335A1 Novel polypeptides and methods for their use 公开/授权日：2003-01-30
信息查询
