发明授权
US06797829B1 Synthesis of a novel paramagnetic amino derivative (epm-5) for labelling chemical and biological macromolecules
失效
用于标记化学和生物大分子的新型顺磁性氨基衍生物（epm-5）的合成
- 专利标题： Synthesis of a novel paramagnetic amino derivative (epm-5) for labelling chemical and biological macromolecules
- 专利标题（中）： 用于标记化学和生物大分子的新型顺磁性氨基衍生物（epm-5）的合成
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申请号： US10018842申请日： 2001-12-21
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公开(公告)号： US06797829B1公开(公告)日： 2004-09-28
- 发明人: Clóvis Ryuichi Nakaie , Mineko Tominaga , Antonio Cechlli Matto Paiva , Simone dos Reis Barbosa , Reinaldo Machetto , Shirley Schreier
- 申请人： Clóvis Ryuichi Nakaie , Mineko Tominaga , Antonio Cechlli Matto Paiva , Simone dos Reis Barbosa , Reinaldo Machetto , Shirley Schreier
- 优先权： BR9903137 19990622
- 主分类号： C07D20746
- IPC分类号： C07D20746
摘要：
The present invention refers to the synthesis and application of 2,2,5,5-tetramethylpyrrolidine-N-oxyl-(9-fluorenylmethyloxycarbonyl)-3-amine-4-carboxylic acid, a novel paramagnetic amino acid derivative (spin label), denominated Fmoc-Poac. Fmoc-Poac can be coupled to peptide sequences and other molecules or systems. It can be inserted anywhere in a peptide segment, even at an internal position if necessary, after removal of its temporary amine protecting group, Fmoc. Owing to its pyrrolidine structure, this molecule may induce differentiated conformations as compared with normal &agr;-amino acids, thus being a valuable probe for structural-biological activity of several relevant peptides. The Poac-angiotensin II analogue was synthesized as a model according to its use as a chemical derivative.
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