发明授权
- 专利标题： Isolation and purification of Clostridium botulinum toxins
- 专利标题（中）： 肉毒杆菌毒素的分离和纯化
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申请号： US10137507申请日： 2002-04-30
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公开(公告)号： US06818409B2公开(公告)日： 2004-11-16
- 发明人: Kenji Oguma
- 申请人： Kenji Oguma
- 优先权： JP2001-202736 20010703
- 主分类号： C07K1433
- IPC分类号： C07K1433
摘要：
A method for separating and purifying HA-positive progenitor toxin(s) (LL and/or L toxins) and HA-negative progenitor toxin (M toxin) from a Clostridium botulinum strain is provided. The method comprises applying a liquid containing both the HA-positive progenitor toxin(s) and the HA-negative progenitor toxin to a lactose column. Also provided is a method for separating and purifying neurotoxin (7S toxin) from HA-positive progenitor toxins, which comprises treating HA-positive progenitor toxins with an alkaline buffer and then applying the resulting liquid containing dissociated neurotoxin and non-toxic components to a lactose column. Activated pure HA-positive toxins (L and LL toxins) and neurotoxin are obtained by simple procedures.