公开(公告)号：US06818409B2

公开(公告)日：2004-11-16

申请号：US10137507

申请日：2002-04-30

申请人： Kenji Oguma

发明人： Kenji Oguma

IPC分类号： C07K1433

CPC分类号： C07K14/33 ,  Y10S435/80 ,  Y10S435/82 ,  Y10S530/82

摘要： A method for separating and purifying HA-positive progenitor toxin(s) (LL and/or L toxins) and HA-negative progenitor toxin (M toxin) from a Clostridium botulinum strain is provided. The method comprises applying a liquid containing both the HA-positive progenitor toxin(s) and the HA-negative progenitor toxin to a lactose column. Also provided is a method for separating and purifying neurotoxin (7S toxin) from HA-positive progenitor toxins, which comprises treating HA-positive progenitor toxins with an alkaline buffer and then applying the resulting liquid containing dissociated neurotoxin and non-toxic components to a lactose column. Activated pure HA-positive toxins (L and LL toxins) and neurotoxin are obtained by simple procedures.

摘要翻译： 提供了从肉毒梭菌菌株分离和纯化HA阳性祖细胞毒素（LL和/或L毒素）和HA阴性祖细胞毒素（M毒素）的方法。 该方法包括将含有HA-阳性祖细胞毒素和HA-阴性祖细胞毒素的液体施用于乳糖柱。 还提供了用于从HA阳性祖细胞毒素分离和纯化神经毒素（7S毒素）的方法，其包括用碱性缓冲液处理HA阳性祖细胞毒素，然后将含有解离的神经毒素和无毒成分的所得液体施用于乳糖 柱。 通过简单的程序获得活化的纯HA-阳性毒素（L和LL毒素）和神经毒素。