发明授权
- 专利标题: Supports for the parallel identification and transcription profiling of polynucleic acids
- 专利标题(中): 支持多核酸的平行鉴定和转录谱
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申请号: US09701584申请日: 2001-02-01
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公开(公告)号: US06830886B1公开(公告)日: 2004-12-14
- 发明人: Andreas Bosio , Wilhelm Stoffel , Markus Stoffel
- 申请人: Andreas Bosio , Wilhelm Stoffel , Markus Stoffel
- 优先权: DE19825899 19980610; EP98110608 19980610
- 主分类号: C12Q168
- IPC分类号: C12Q168
摘要:
The invention relates to a support. Oligonucleotides or polynucleotides are covalently bound with the 5′- or 3′-termination on least one main surface of said support via bifunctional spacers and bifunctional linkers. The support is characterized in that the oligonucleotides or polynucleotides which are covalently bound with the 5′- or 3′-termination via bifunctional spacers and bifunctional linkers comprise 200 to 600 bp, and the oligonucleotides or polynucleotides can be obtained by using a method which comprises the following steps: Selecting homologous regions of mRNA of a target species and of at least one model species; selecting amplification primers which permit the amplification of 200 to 600, preferably 200 to 400 bp long nucleic acids from the homologous regions of both the mRNA of the target species and the mRNA of at least one model species, whereby the amplification primers optionally comprise a maximum of 1 mismatch per 6 nucleic acids of the amplification primer; immobilizing the nucleic acids on at the least one main surface of the support, said nucleic acids being obtained from the corresponding 200 to 600 bp long nucleic acids which are amplified for the target species or for the at least one model species by amplifications using the amplification primers.
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