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    Supports for the parallel identification and transcription profiling of polynucleic acids
    1.
    发明授权
    Supports for the parallel identification and transcription profiling of polynucleic acids 失效
    支持多核酸的平行鉴定和转录谱

    公开(公告)号：US06830886B1

    公开(公告)日：2004-12-14

    申请号：US09701584

    申请日：2001-02-01

    申请人： Andreas Bosio ,  Wilhelm Stoffel ,  Markus Stoffel

    发明人： Andreas Bosio ,  Wilhelm Stoffel ,  Markus Stoffel

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6834 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00722 ,  C07B2200/11 ,  C07H21/00 ,  C40B40/06

    摘要： The invention relates to a support. Oligonucleotides or polynucleotides are covalently bound with the 5′- or 3′-termination on least one main surface of said support via bifunctional spacers and bifunctional linkers. The support is characterized in that the oligonucleotides or polynucleotides which are covalently bound with the 5′- or 3′-termination via bifunctional spacers and bifunctional linkers comprise 200 to 600 bp, and the oligonucleotides or polynucleotides can be obtained by using a method which comprises the following steps: Selecting homologous regions of mRNA of a target species and of at least one model species; selecting amplification primers which permit the amplification of 200 to 600, preferably 200 to 400 bp long nucleic acids from the homologous regions of both the mRNA of the target species and the mRNA of at least one model species, whereby the amplification primers optionally comprise a maximum of 1 mismatch per 6 nucleic acids of the amplification primer; immobilizing the nucleic acids on at the least one main surface of the support, said nucleic acids being obtained from the corresponding 200 to 600 bp long nucleic acids which are amplified for the target species or for the at least one model species by amplifications using the amplification primers.

    摘要翻译： 本发明涉及一种支撑体。 寡核苷酸或多核苷酸通过双官能间隔基和双官能连接体与所述载体的至少一个主表面上的5'或3'端基共价结合。 该载体的特征在于通过双功能间隔基和双官能连接基与5'-或3'末端共价结合的寡核苷酸或多核苷酸包含200至600bp，并且寡核苷酸或多核苷酸可以通过使用包含 以下步骤：选择目标物种和至少一种模式物种的mRNA的同源区; 选择允许从目标物种的mRNA的同源区和至少一个模型物种的mRNA的同源区扩增200至600个，优选200至400bp长的核酸的扩增引物，由此扩增引物任选地包含最大 每扩增引物6个核酸1个错配; 将核酸固定在载体的至少一个主表面上，所述核酸从相应的200至600bp长的核酸获得，所述核酸通过扩增用于靶物种或至少一种模型物种扩增 引物。

    Synthetic tear fluid
    3.
    发明申请
    Synthetic tear fluid 审中-公开
    合成泪液

    公开(公告)号：US20060177402A1

    公开(公告)日：2006-08-10

    申请号：US11218720

    申请日：2005-09-06

    申请人： Wilhelm Stoffel

    发明人： Wilhelm Stoffel

    IPC分类号： A61K31/7008 ,  A61K31/685

    CPC分类号： A61K9/127 ,  A61K9/0048 ,  A61K47/24

    摘要： Synthetic tear fluid comprising at least one glycerophospholipids selected from the group consisting of phosphatidylcholines, phosphatidylserines, phosphatidylethanolamines, and phosphatidylinositol, cholesterol in a glycerophospholipid:cholesterol molar ratio up to 1:1 at least one lipophilic ionic compound, preferably a ganglioside, in a glycerophospholipid:lipophilic ionic compound molar ratio of 100:1 to 10:1 at least one buffer substance water.

    摘要翻译： 包含至少一种选自磷脂酰胆碱，磷脂酰丝氨酸，磷脂酰乙醇胺和磷脂酰肌醇的甘油磷脂的合成泪液，甘油磷脂中的胆固醇：高达1:1的胆固醇摩尔比在甘油磷脂中至少一种亲脂性离子化合物，优选神经节苷脂 ：亲脂性离子化合物的摩尔比为100：1至10：1，至少一种缓冲物质的水。

    Lipohaptens comprising a multifunctional substituted lipohilic carrier linked to an antigen and their use
    4.
    发明授权
    Lipohaptens comprising a multifunctional substituted lipohilic carrier linked to an antigen and their use 失效
    Lipohaptens包含与抗原连接的多功能取代的脂质载体及其用途

    公开(公告)号：US5770390A

    公开(公告)日：1998-06-23

    申请号：US621050

    申请日：1996-03-22

    申请人： Wilhelm Stoffel

    发明人： Wilhelm Stoffel

    IPC分类号： G01N33/531 ,  G01N33/543 ,  G01N33/92 ,  G01N33/545

    CPC分类号： G01N33/54306 ,  G01N33/531 ,  G01N33/92 ,  Y10S436/822

    摘要： Lipohaptens of the general formula I (X).sub.m --R--Y--(S).sub.n --P (I) wherein R is a hydrocarbyl backbone having from 2 to 30 carbon atoms X is a hydrocarbyl radical having from 6 to 30 carbon atoms, which is linked to R via a bonding group containing one or more heteroatoms m is an integer from 1 to 5 P is a polypeptide or polysaccharide residue having from 2 to 100 aminoacid or glycosyl units Y is a functional group capable of binding to a free amino group of the polypeptide P or a carboxy, hemiacetal or hydroxy group of the polysaccharide P S is a difunctional spacer group, the functional groups thereof being capable of binding with the functional group Y on the one hand and a free amino, carboxy or hydroxy group of the polypeptide or polysaccaride P on the other hand, and n is 0 or 1 and their use in immunoreactions for determining antibodies and antigens, respectively.

    摘要翻译： 通式I（X）mry-（S）nP（I）的通式（I）的巯基联苯，其中R是具有2-30个碳原子的烃基主链X是具有6至30个碳原子的烃基，其与R通 含有一个或多个杂原子的键合基团是1至5的整数.P是具有2至100个氨基酸或糖基单元的多肽或多糖残基Y是能够结合多肽P的游离氨基的官能团或 多糖PS的羧基，半缩醛或羟基是双官能间隔基团，其功能基团一方面可以与官能团Y和多肽或多糖的P的游离氨基，羧基或羟基结合 另一方面，n为0或1，它们分别用于免疫反应以确定抗体和抗原。