发明授权
- 专利标题: Method for extracting an insulin using enzymes which are bonded to a non-porous polymer support
- 专利标题(中): 使用与无孔聚合物载体结合的酶提取胰岛素的方法
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申请号: US09700391申请日: 1999-05-03
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公开(公告)号: US06852513B1公开(公告)日: 2005-02-08
- 发明人: Jürgen Bongs , Johannes Meiwes
- 申请人: Jürgen Bongs , Johannes Meiwes
- 申请人地址: DE Frankfurt am Main
- 专利权人: Aventis Pharma Deutschland GmbH
- 当前专利权人: Aventis Pharma Deutschland GmbH
- 当前专利权人地址: DE Frankfurt am Main
- 代理机构: Finnegan, Henderson, Farabow, Garrett, & Dunner L.L.P.
- 优先权: DE19821866 19980515
- 国际申请: PCTEP99/02973 WO 19990503
- 国际公布: WO9960150 WO 19991125
- 主分类号: C12N11/00
- IPC分类号: C12N11/00 ; C07K1/04 ; C07K1/12 ; C07K14/62 ; C12N11/02 ; C12N11/06 ; C12N11/08 ; C12P1/00 ; C12P19/00 ; C12P21/00 ; C12P21/02 ; C12P21/06 ; C12N15/09
摘要:
The invention relates to a method for catalysing complex reactions or large molecules, more specifically, to enzyme-catalysed reactions during which undesirable consecutive or subsidiary reactions usually occur; using enzymes which are bonded to a polymer support. According to the invention, undesirable consecutive or subsidiary reactions are to a large extent avoided by selecting a non-porous or almost non-porous support material. In particular, the invention relates to a method for the enzymatic extraction of biomolecules, preferably peptides, proteins, oligosaccharides or polysaccharides from their biologically inactive precursors using enzymes which are bonded to a polymer support, especially a method for extracting insulins or their analogs from the corresponding precursors using enzymes which are bonded to a polymer support. As a result of selecting a non-porous almost non-porous support material, this method leads to a selective formation of biomolecules, especially of insulins or insulin analogs and corresponding valuable substances which can be split into said insulins or their analogs, undesirable consecutive or subsidiary reactions being to a large extent avoided.