发明授权
- 专利标题: Recombinant negative strand RNA virus expression systems and vaccines
- 专利标题(中): 重组负链RNA病毒表达系统和疫苗
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申请号: US09396539申请日: 1999-09-14
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公开(公告)号: US06887699B1公开(公告)日: 2005-05-03
- 发明人: Peter Palese , Adolfo Garcia-Sastre
- 申请人: Peter Palese , Adolfo Garcia-Sastre
- 申请人地址: US CA Mountain View
- 专利权人: MedImmune Vaccines, Inc.
- 当前专利权人: MedImmune Vaccines, Inc.
- 当前专利权人地址: US CA Mountain View
- 代理机构: Quine Intellectual Property Law Group, P.C.
- 代理商 Paul Littlepage; Jonathan Klein-Evans
- 主分类号: A61K39/00
- IPC分类号: A61K39/00 ; C07K14/11 ; C07K14/16 ; C12N9/12 ; C12N9/24 ; C12N15/10 ; C12N15/11 ; C12N15/86 ; C12N7/02
摘要:
Recombinant negative-strand viral RNA templates are described which may be used with purified RNA-directed RNA polymerase complex to express heterologous gene products in appropriate host cells and/or to rescue the heterologous gene in virus particles. The RNA templates are prepared by transcription of appropriate DNA sequences with a DNA-directed RNA polymerase. The resulting RNA templates are of the negative-polarity and contain appropriate terminal sequences which enable the viral RNA-synthesizing apparatus to recognize the template. Bicistronic mRNAs can be constructed to permit internal initiation of translation of viral sequences and allow for the expression of foreign protein coding sequences from the regular terminal initiation site, or vice versa.
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