发明授权
- 专利标题： Dna encoding sucrose pts enzyme II
- 专利标题（中）： Dna编码蔗糖酶Ⅱ
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申请号： US10019284申请日： 2000-06-30
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公开(公告)号： US06893852B1公开(公告)日： 2005-05-17
- 发明人: Masako Izui , Masakazu Sugimoto , Tsuyoshi Nakamatsu , Osamu Kurahashi
- 申请人： Masako Izui , Masakazu Sugimoto , Tsuyoshi Nakamatsu , Osamu Kurahashi
- 申请人地址： JP Tokyo
- 专利权人： Ajinomoto Co., Inc.
- 当前专利权人： Ajinomoto Co., Inc.
- 当前专利权人地址： JP Tokyo
- 代理商 Shelly Guest Cermak
- 优先权： JP11-189512 19990702
- 国际申请： PCTJP00/04348 WO 20000630
- 国际公布： WO0102584 WO 20010111
- 主分类号： C12N9/12
- IPC分类号： C12N9/12 ; C12N15/54 ; C07H21/04
摘要：
A gene encoding a protein constituting sucrose PTS of coryneform bacterium is provided by amplifying a region existing downstream from sucrase gene in coryneform bacterium by the cassette-ligation mediated PCR to obtain DNA encoding sucrose PTS enzyme II, which is a protein defined in the following (A) or (B): (A) a protein which has the amino acid sequence of SEQ ID NO: 2 in Sequence Listing; (B) a protein which has the amino acid sequence of SEQ ID NO: 2 in Sequence Listing including substitution, deletion, insertion, addition or inversion of one or several amino acids, and an activity for binding to sucrose.
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