发明授权
- 专利标题: Controlled reversible poration for preservation of biological materials
- 专利标题(中): 用于保存生物材料的可控可逆定点
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申请号: US09443842申请日: 1999-11-19
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公开(公告)号: US06902931B1公开(公告)日: 2005-06-07
- 发明人: Mehmet Toner , Michael Russo , Robert Bieganski
- 申请人: Mehmet Toner , Michael Russo , Robert Bieganski
- 申请人地址: US MA Boston
- 专利权人: The General Hospital Coporation
- 当前专利权人: The General Hospital Coporation
- 当前专利权人地址: US MA Boston
- 代理机构: Nutter McClennen & Fish
- 主分类号: A01N1/02
- IPC分类号: A01N1/02 ; C12N5/00 ; C12N5/06 ; C12N5/08
摘要:
A preservation method for biological material having cell membranes includes reversibly porating the cell membranes; loading a bio-protective agent having bio-preservation properties to a predetermined intracellular concentration; preparing the bio-protective agent loaded biological material for storage; storing the biological material; recovering the stored biological material from storage; and reversing the cell membrane poration. H5 α-toxin, a genetically engineered mutant of Staphylococcus aureus α-hemolysin, may be used as a porating agent. Non-permeating sugars such as trehalose and sucrose may be used as the bio-protective agent.