发明授权
- 专利标题： High fidelity thermostable ligase and uses thereof
- 专利标题（中）： 高保真热稳定连接酶及其用途
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申请号： US09830502申请日： 1999-10-29
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公开(公告)号： US06949370B1公开(公告)日： 2005-09-27
- 发明人: Francis Barany , Weiguo Cao , Jie Tong
- 申请人： Francis Barany , Weiguo Cao , Jie Tong
- 申请人地址： US NY Ithaca
- 专利权人： Cornell Research Foundation, Inc.
- 当前专利权人： Cornell Research Foundation, Inc.
- 当前专利权人地址： US NY Ithaca
- 代理机构： Nixon Peabody LLP
- 国际申请： PCT/US99/25437 WO 19991029
- 国际公布： WO00/26381 WO 20000511
- 主分类号： C07H21/04
- IPC分类号： C07H21/04 ; C12N9/00 ; C12N9/22 ; C12Q1/68
摘要：
The present invention is directed to a thermostable ligase having substantially higher fidelity than either T4 ligase or Thermus thermophilus ligase. The DNA molecule encoding this enzyme as well as expression systems and host cells containing it are also disclosed. The thermostable ligase of the present invention is useful in carrying out a ligase detection reaction process and a ligase chain reaction process.