发明授权
- 专利标题： Synthetic molecules for labeling histidine-rich proteins
- 专利标题（中）： 用于标记富含组氨酸的蛋白质的合成分子
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申请号： US10759762申请日： 2004-01-16
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公开(公告)号： US07041821B2公开(公告)日： 2006-05-09
- 发明人: Roger Y. Tsien , Christina Hauser
- 申请人： Roger Y. Tsien , Christina Hauser
- 申请人地址： US CA Oakland
- 专利权人： The Regents of the University of California
- 当前专利权人： The Regents of the University of California
- 当前专利权人地址： US CA Oakland
- 代理机构： Townsend and Townsend and Crew LLP
- 主分类号： C07D265/38
- IPC分类号： C07D265/38 ; C07D311/80
摘要：
The invention provides Zn-chelating compounds that are molecularly engineered to bind to a specific target sequence in a protein of interest. The Zn2+ ion is far less toxic and promiscuous than nickel and therefore provides an attractive alternative to Ni-based labeling systems. Invention Zn-chelating compounds also do not require oxidizable thiols and therefore can be used in non-reducing environments such as the surface of living cells. In addition, the target sequence is genetically encodable and requires incorporation of only a few amino acids, unlike fusions to fluorescent proteins such as GFP.
公开/授权文献
- US20040241749A1 Synthetic molecules for labeling histidine-rich proteins 公开/授权日：2004-12-02
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