发明授权
- 专利标题: Method of reusing DNA-immobilization substrate
- 专利标题(中): 重复使用DNA固定底物的方法
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申请号: US10451399申请日: 2001-12-17
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公开(公告)号: US07078517B2公开(公告)日: 2006-07-18
- 发明人: Kojiro Takahashi , Osamu Takai , Michifumi Tanga
- 申请人: Kojiro Takahashi , Osamu Takai , Michifumi Tanga
- 申请人地址: JP Tokyo JP Hiroshima JP Hiroshima
- 专利权人: Toyo Kohan Co., LTD,Nihon Parrkerizing Hiroshima Co., LTD,Tojiro Takahashi
- 当前专利权人: Toyo Kohan Co., LTD,Nihon Parrkerizing Hiroshima Co., LTD,Tojiro Takahashi
- 当前专利权人地址: JP Tokyo JP Hiroshima JP Hiroshima
- 代理机构: Browdy and Neimark, PLLC
- 优先权: JP2000-401624 20001228
- 国际申请: PCT/JP01/11027 WO 20011217
- 国际公布: WO02/054068 WO 20020711
- 主分类号: C07H23/00
- IPC分类号: C07H23/00
摘要:
It is intended to provide a method of reusing a DNA-immobilization substrate whereby the expensive DNA-immobilization substrate can be efficiently utilized and a reusable DNA-immobilization substrate having the same performance as a new product without any trouble in practical use can be provided. Namely, a method of reusing a DNA-immobilization substrate characterized in that, to remove DNA from a DNA-immobilization substrate carrying the DNA immobilized by an acid-amide bond via an oligonucleotide to thereby enable the immobilization of a fresh DNA, the acid-amide bond between the substrate and the DNA is hydrolyzed with an acid or an alkali.
公开/授权文献
- US20040092005A1 Method of reusing dna-immobilization substrate 公开/授权日:2004-05-13
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