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23 条记录 (耗时 0.012 秒)

    Method of reusing DNA-immobilization substrate
    2.
    发明授权
    Method of reusing DNA-immobilization substrate 失效
    重复使用DNA固定底物的方法

    公开(公告)号：US07078517B2

    公开(公告)日：2006-07-18

    申请号：US10451399

    申请日：2001-12-17

    申请人： Kojiro Takahashi ,  Osamu Takai ,  Michifumi Tanga

    发明人： Kojiro Takahashi ,  Osamu Takai ,  Michifumi Tanga

    IPC分类号： C07H23/00

    CPC分类号： C07H21/00 ,  B01J2219/00529 ,  B01J2219/00608 ,  C40B99/00

    摘要： It is intended to provide a method of reusing a DNA-immobilization substrate whereby the expensive DNA-immobilization substrate can be efficiently utilized and a reusable DNA-immobilization substrate having the same performance as a new product without any trouble in practical use can be provided. Namely, a method of reusing a DNA-immobilization substrate characterized in that, to remove DNA from a DNA-immobilization substrate carrying the DNA immobilized by an acid-amide bond via an oligonucleotide to thereby enable the immobilization of a fresh DNA, the acid-amide bond between the substrate and the DNA is hydrolyzed with an acid or an alkali.

    摘要翻译： 旨在提供一种重复使用DNA固定化底物的方法，由此可以有效地利用昂贵的DNA固定化底物，并且可以提供具有与新产品相同性能的可再利用的DNA固定基质，而无需实际使用中的任何麻烦。 即，重复利用DNA固定基质的方法，其特征在于，为了从DNA固定化载体中除去DNA，通过寡核苷酸携带由酰胺酰胺键固定的DNA，从而能够固定新鲜的DNA， 底物与DNA之间的酰胺键用酸或碱水解。

    Articles with diamond coating formed thereon by vapor-phase synthesis
    3.
    发明授权
    Articles with diamond coating formed thereon by vapor-phase synthesis 失效
    通过气相合成在其上形成金刚石涂层的制品

    公开(公告)号：US5935323A

    公开(公告)日：1999-08-10

    申请号：US945212

    申请日：1998-03-20

    申请人： Michifumi Tanga ,  Takahiro Kitagawa

    发明人： Michifumi Tanga ,  Takahiro Kitagawa

    IPC分类号： C23C16/02 ,  C23C16/26 ,  C23C16/27 ,  C30B29/04

    CPC分类号： C23C16/0263 ,  C23C16/0254 ,  C23C16/26 ,  C23C16/27

    摘要： Articles with a tenaciously adherent diamond coating are made by forming a diamond coating on a base material by vapor-phase synthesis without causing any warpage of the coating. The diamond coating layer is formed on the surface of a base material having a number of pores formed by electric discharge or laser beams and having a depth of 0.0001-0.2 mm and a diameter of 0.001-0.02 mm. The pores may be connected to one another to form a groove. Suitable examples of the base material include molybdenum, tungsten, silicon, tungsten carbide, silicon carbide, silicon nitride, and cemented carbide mainly comprising tungsten carbide and cobalt and/or nickel.

    摘要翻译： PCT No.PCT / JP96 / 01052 Sec。 371日期：1998年3月20日 102（e）1998年3月20日PCT PCT 1996年4月18日PCT公布。 公开号WO96 / 34131 日期1996年10月31日具有强力粘附金刚石涂层的文章通过气相合成在基材上形成金刚石涂层而不会引起涂层翘曲而制成。 金刚石涂层形成在具有多个由放电或激光束形成的孔的基材的表面上，其深度为0.0001-0.2mm，直径为0.001-0.02mm。 孔可以彼此连接以形成凹槽。 基材的合适实例包括钼，钨，硅，碳化钨，碳化硅，氮化硅和主要包含碳化钨和钴和/或镍的硬质合金。

    Process for removing polymer scale adhering to a polymerization vessel
    4.
    发明授权
    Process for removing polymer scale adhering to a polymerization vessel 失效
    用于去除聚合容器附着的聚合物垢的方法

    公开(公告)号：US4517350A

    公开(公告)日：1985-05-14

    申请号：US604751

    申请日：1984-04-27

    申请人： Akio Hata ,  Michifumi Tanga

    发明人： Akio Hata ,  Michifumi Tanga

    IPC分类号： C08F14/00 ,  C08F2/00 ,  C08F6/00 ,  C08F6/06 ,  C08F14/06 ,  C08F114/06

    CPC分类号： C08F2/004

    摘要： A method for removing polymer scales adhering to the inner surface of a polymerization vessel, which comprises polymerizing vinyl chloride monomer in an aqueous medium, discharging the resulting vinyl chloride polymer together with the aqueous medium from the polymerization vessel, thereafter placing in the vessel both a reaction product resulting from the reaction of an amine and acrylic acid or its derivative and an organic liquid which can dissolve or swell the polyvinyl polymer, and washing the inner surface of the vessel with the organic liquid solution of the reaction product.

    摘要翻译： 一种除去聚合容器内表面附着的聚合物垢的方法，该方法包括在水性介质中聚合氯乙烯单体，将聚合物与聚合容器一起排出所得的氯乙烯聚合物，然后将容器中的 由胺和丙烯酸或其衍生物反应产生的反应产物和可溶解或溶胀聚乙烯基聚合物的有机液体，并用反应产物的有机液体溶液洗涤容器的内表面。

    Method and unit for detection of interactions of biologically relevant molecules
    7.
    发明授权
    Method and unit for detection of interactions of biologically relevant molecules 有权
    用于检测生物相关分子相互作用的方法和单元

    公开(公告)号：US08778667B2

    公开(公告)日：2014-07-15

    申请号：US12994990

    申请日：2009-05-26

    申请人： Hirofumi Yamano ,  Shuuichi Kamei ,  Michifumi Tanga

    发明人： Hirofumi Yamano ,  Shuuichi Kamei ,  Michifumi Tanga

    IPC分类号： C12M1/34

    CPC分类号： B01L3/50825 ,  B01L2200/142 ,  B01L2300/042 ,  B01L2300/046 ,  B01L2300/0636 ,  G01N33/54386

    摘要： The present invention relates to a unit to be used for detection of interactions of biologically relevant molecules using a carrier on which the biologically relevant molecules are immobilized, comprising: a hollow holder having an open part at one end with the other end being closed; and a carrier-supporting member that can be inserted into the hollow holder, on which is mounted a carrier upon which biologically relevant molecules are immobilized, wherein: while the carrier-supporting member is being inserted into the hollow holder, a rear-end portion of the carrier-supporting member is engaged with an edge of the open part of the hollow holder, so that the hollow holder is sealed and the positions of the carrier-supporting member and the hollow holder are determined; and the area on the left and the area on the right of the axial center, which are defined by the inner side of the hollow holder and the external side of the carrier-supporting member on which the carrier has been mounted, are approximately the same within the region from the carrier-mounting part to the apical part of the carrier-supporting member, as in a section cut along a plane including the axial center of the hollow holder in a positioned state.

    摘要翻译： 本发明涉及一种用于检测生物相关分子的相互作用的单元，所述分子使用其上固定有生物相关分子的载体，包括：中空保持器，其一端具有开放部分，另一端封闭; 以及载体支撑构件，其能够插入到所述中空保持器中，所述载体支撑构件安装在固定有生物相关分子的载体上，其中：当所述载体支撑构件插入所述中空保持器中时，后端部 载体支撑构件与中空保持器的开口部分的边缘接合，使得中空保持器被密封，并且确定载体支撑构件和中空保持器的位置; 并且由中空保持器的内侧和其上安装有载体的载体支撑构件的外侧限定的左轴和中心的左侧区域和右侧的区域大​​致相同 在从载体安装部到载体支撑构件的顶部的区域内，如在包括处于定位状态的中空保持器的轴心的平面的截面中。

    Support for fixing nucleotide and process for producing the same
    9.
    发明申请
    Support for fixing nucleotide and process for producing the same 审中-公开
    支持固定核苷酸及其制备方法

    公开(公告)号：US20060024741A1

    公开(公告)日：2006-02-02

    申请号：US11239418

    申请日：2005-09-30

    申请人： Michifumi Tanga ,  Hiroshi Okamura ,  Kenichi Takagi ,  Kojiro Takahashi

    发明人： Michifumi Tanga ,  Hiroshi Okamura ,  Kenichi Takagi ,  Kojiro Takahashi

    IPC分类号： C40B40/08 ,  C40B50/18

    CPC分类号： C07H21/00 ,  C07B2200/11 ,  C12N15/1006 ,  C12Q1/6837 ,  C12Q2521/501 ,  C12Q2521/301

    摘要： A support for fixing a nucleotide that contributes to the efficient clarification of DNA without damaging the terminal parts of the DNA and, therefore, is useful in the fields of biology, biochemistry and the like. This support for fixing a nucleotide is a chemically modified substrate on which oligonucleotide are immobilized characterized in that the oligonucleotide have a restriction enzyme cleavage site. This support is produced by chemically modifying the substrate, immobilizing a single-stranded oligonucleotide thereon, then hybridizing the single-stranded oligonucleotide with another single-stranded oligonucleotide having a base sequence complementary thereto, and ligating an oligonucleotide having a restriction enzyme site at the end thereof.

    摘要翻译： 用于固定有助于DNA的有效澄清而不损害DNA的末端部分的核苷酸的支持物，因此可用于生物学，生物化学等领域。 用于固定核苷酸的这种支持是其上固定有寡核苷酸的化学修饰底物，其特征在于寡核苷酸具有限制酶切割位点。 通过化学修饰底物，将单链寡核苷酸固定在其上，然后将单链寡核苷酸与其碱基序列互补的另一单链寡核苷酸杂交，并在末端连接具有限制酶位点的寡核苷酸， 其中。