- 专利标题： Composition and method for hot start nucleic acid amplification
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申请号： US10192902申请日： 2002-07-11
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公开(公告)号： US07122355B2公开(公告)日： 2006-10-17
- 发明人: Waltraud Ankenbauer , Dieter Heindl , Frank Laue , Andreas Huber
- 申请人： Waltraud Ankenbauer , Dieter Heindl , Frank Laue , Andreas Huber
- 申请人地址： US IN Indianapolis
- 专利权人： Roche Diagnostics Operations, Inc.
- 当前专利权人： Roche Diagnostics Operations, Inc.
- 当前专利权人地址： US IN Indianapolis
- 代理机构： Roche Diagnostics Operations, Inc.
- 代理商 Marilyn L. Amick
- 优先权： EP01115788 20010711
- 主分类号： C12P19/34
- IPC分类号： C12P19/34
摘要：
The present invention is directed to a new composition for performing a nucleic acid amplification reaction comprising (i) a thermostable DNA-Polymerase, (ii) a thermostable 3′-5′ Exonuclease, and (iii) at least one primer for nucleic acid amplification with a modified 3′ terminal residue which is not elongated by said thermostable DNA-Polymerase as well as methods for performing a PCR reaction using this composition. Furthermore, the method is directed to kits comprising such a composition.
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