发明授权
- 专利标题： Method of detecting a nucleic acid using a fluorescent group-containing carbodiimide as a label
- 专利标题（中）： 使用含荧光基的碳二亚胺作为标记检测核酸的方法
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申请号： US10654305申请日： 2003-09-02
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公开(公告)号： US07135293B2公开(公告)日： 2006-11-14
- 发明人: Naoki Kimura , Namiko Shiohata , Yoko Yoshikawa
- 申请人： Naoki Kimura , Namiko Shiohata , Yoko Yoshikawa
- 申请人地址： JP Tokyo
- 专利权人： Nisshinbo Industries, Inc.
- 当前专利权人： Nisshinbo Industries, Inc.
- 当前专利权人地址： JP Tokyo
- 代理机构： Knobbe, Martens, Olson & Bear, LLP
- 优先权： JP11-81666 19990325; JP11-284107 19991005
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
A novel fluorescent group-containing carbodiimide compound having at least one group selected from a carboxyl group, a sulfo group, a phosphono group and a phospho group which have substitution of an alkali metal, an alkaline earth metal or a basic group containing a nitrogen or phosphorus atom, which is prepared by using a novel fluorescent group-containing carbodiimide compound precursor having a halogen atom or a sulfonic acid group; and a method for detecting a nucleic acid by hybridization utilizing a nucleic acid labeled with a labeling substance, which utilizes the fluorescent group-containing carbodiimide compound as the labeling substance.
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