公开(公告)号：US07176330B2

公开(公告)日：2007-02-13

申请号：US10654363

申请日：2003-09-02

申请人： Naoki Kimura ,  Namiko Shiohata ,  Yoko Yoshikawa

发明人： Naoki Kimura ,  Namiko Shiohata ,  Yoko Yoshikawa

IPC分类号： C07D265/30 ,  C07D249/00

CPC分类号： C09K11/06 ,  C09K2211/1007 ,  C09K2211/1011 ,  C09K2211/1014 ,  C09K2211/1029 ,  C09K2211/1033 ,  C09K2211/1088 ,  Y10T436/143333

摘要： A novel fluorescent group-containing carbodiimide compound having at least one group selected from a carboxyl group, a sulfo group, a phosphono group and a phospho group which have substitution of an alkali metal, an alkaline earth metal or a basic group containing a nitrogen or phosphorus atom, which is prepared by using a novel fluorescent group-containing carbodiimide compound precursor having a halogen atom or a sulfonic acid group; and a method for detecting a nucleic acid by hybridization utilizing a nucleic acid labeled with a labeling substance, which utilizes the fluorescent group-containing carbodiimide compound as the labeling substance.

摘要翻译： 具有选自羧基，磺基，膦酰基和磷酸基中的至少一个基团的含荧光基团的碳二亚胺化合物，其具有碱金属，碱土金属或含氮的碱性基团的取代基，或 磷原子，其通过使用具有卤素原子或磺酸基的新的含有荧光团的碳二亚胺化合物前体制备; 以及利用利用含荧光基的碳二亚胺化合物作为标记物质的用标记物质标记的核酸杂交来检测核酸的方法。

公开(公告)号：US07135293B2

公开(公告)日：2006-11-14

申请号：US10654305

申请日：2003-09-02

申请人： Naoki Kimura ,  Namiko Shiohata ,  Yoko Yoshikawa

发明人： Naoki Kimura ,  Namiko Shiohata ,  Yoko Yoshikawa

IPC分类号： C12Q1/68

CPC分类号： C09K11/06 ,  C09K2211/1007 ,  C09K2211/1011 ,  C09K2211/1014 ,  C09K2211/1029 ,  C09K2211/1033 ,  C09K2211/1088 ,  Y10T436/143333

摘要： A novel fluorescent group-containing carbodiimide compound having at least one group selected from a carboxyl group, a sulfo group, a phosphono group and a phospho group which have substitution of an alkali metal, an alkaline earth metal or a basic group containing a nitrogen or phosphorus atom, which is prepared by using a novel fluorescent group-containing carbodiimide compound precursor having a halogen atom or a sulfonic acid group; and a method for detecting a nucleic acid by hybridization utilizing a nucleic acid labeled with a labeling substance, which utilizes the fluorescent group-containing carbodiimide compound as the labeling substance.

摘要翻译： 具有选自羧基，磺基，膦酰基和磷酸基中的至少一个基团的含荧光基团的碳二亚胺化合物，其具有碱金属，碱土金属或含氮的碱性基团的取代基，或 磷原子，其通过使用具有卤素原子或磺酸基的新的含有荧光团的碳二亚胺化合物前体制备; 以及利用利用含荧光基的碳二亚胺化合物作为标记物质的用标记物质标记的核酸杂交来检测核酸的方法。

公开(公告)号：US06642380B1

公开(公告)日：2003-11-04

申请号：US09533918

申请日：2000-03-23

申请人： Naoki Kimura ,  Namiko Shiohata ,  Yoko Yoshikawa

发明人： Naoki Kimura ,  Namiko Shiohata ,  Yoko Yoshikawa

IPC分类号： C07C26700

CPC分类号： C09K11/06 ,  C09K2211/1007 ,  C09K2211/1011 ,  C09K2211/1014 ,  C09K2211/1029 ,  C09K2211/1033 ,  C09K2211/1088 ,  Y10T436/143333

摘要： A novel fluorescent group-containing carbodiimide compound having at least one group selected from a carboxyl group, a sulfo group, a phosphono group and a phospho group which have substitution of an alkali metal, an alkaline earth metal or a basic group containing a nitrogen or phosphorus atom, which is prepared by using a novel fluorescent group-containing carbodiimide compound precursor having a halogen atom or a sulfonic acid group.

摘要翻译： 具有选自羧基，磺基，膦酰基和磷酸基中的至少一个基团的含荧光基团的碳二亚胺化合物，其具有碱金属，碱土金属或含氮的碱性基团的取代基，或 磷原子，其通过使用具有卤素原子或磺酸基团的新的具有荧光基团的碳二亚胺化合物前体制备。