A guiding and positioning apparatus for accurate guiding and input of a print media sheet to a business machine. The guiding and positioning apparatus includes a rotating shaft, a retaining member, a guiding member, a sliding member, a roller, a first spring, a second spring, a tray and a positioning element. The rotating shaft has a protrusion, a first end and a second end. The retaining member is fitted on the first end. The guiding member is fitted on the second end. The sliding member is fitted on the rotating shaft. The sliding member has a sliding groove and a guiding groove. The roller is fitted on the rotating shaft. The first spring is disposed between the retaining member and roller. The second spring is disposed between the roller and sliding member. The tray is disposed under the roller. The positioning element is formed on one side of the tray.