Invention Grant
- Patent Title: Bead-based detection of ligand-GPCR-G protein complexes
- Patent Title (中): 基于珠粒的配体-GPCR-G蛋白复合物的检测
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Application No.: US10429042Application Date: 2003-05-05
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Publication No.: US07189519B2Publication Date: 2007-03-13
- Inventor: Larry A. Sklar , Tione Buranda , Daniel Cimino , Alex T. Key , Richard Neubig , Peter C. Simons , Eric R. Prossnitz , Mei Shi
- Applicant: Larry A. Sklar , Tione Buranda , Daniel Cimino , Alex T. Key , Richard Neubig , Peter C. Simons , Eric R. Prossnitz , Mei Shi
- Applicant Address: US NM Albuquerque US MI Ann Arbor
- Assignee: STC.UNM,Univeristy of Michigan
- Current Assignee: STC.UNM,Univeristy of Michigan
- Current Assignee Address: US NM Albuquerque US MI Ann Arbor
- Agent Henry D. Coleman; R. Neil Sudol; William J. Sapone
- Main IPC: G01N33/52
- IPC: G01N33/52 ; G01N33/566 ; C12N1/20 ; C07K1/00 ; C07H21/04
Abstract:
The present invention provides a general approach for G protein coupled receptors that may be used to define agonists and antagonists, and the specificity of receptor coupling to G protein subunits. Methods of the present invention use small volumes (microliters) and are compatible with high throughput flow cytometry. When assays of the present invention are multiplexed, the specificity of the interactions of a receptor with many G proteins may be determined simultaneously.
Public/Granted literature
- US20030235863A1 Bead-based detection of ligand-GPCR-G protein complexes Public/Granted day:2003-12-25
Information query
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