发明授权
US07214520B2 Cloning, sequencing and expression of a comamonas cyclopentanone 1,2-monooxygenase-encoding gene in Escherichia coli
失效
在大肠杆菌中克隆，测序和表达环戊烯酮-1,2-单加氧酶基因
- 专利标题： Cloning, sequencing and expression of a comamonas cyclopentanone 1,2-monooxygenase-encoding gene in Escherichia coli
- 专利标题（中）： 在大肠杆菌中克隆，测序和表达环戊烯酮-1,2-单加氧酶基因
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申请号： US10312585申请日： 2001-07-13
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公开(公告)号： US07214520B2公开(公告)日： 2007-05-08
- 发明人: Hiroaki Iwaki , Yoshie Hasegawa , Peter C. K. Lau
- 申请人： Hiroaki Iwaki , Yoshie Hasegawa , Peter C. K. Lau
- 申请人地址： CA Ottawa, Ontario
- 专利权人： National Research Council of Canada
- 当前专利权人： National Research Council of Canada
- 当前专利权人地址： CA Ottawa, Ontario
- 代理机构： Ogilvy Renault LLP
- 国际申请： PCT/CA01/01032 WO 20010713
- 国际公布： WO02/06452 WO 20020124
- 主分类号： C12N9/02
- IPC分类号： C12N9/02 ; C12N15/70 ; C12N1/21 ; C12N15/03 ; C12Q1/68 ; C12P21/06 ; C07H21/04
摘要：
Cyclopentanone 1,2-monooxygenase (CPMO) from Comamonas (previously Pseudomonas) sp. strain NCIMB 9872 carries out the second step of a degradation pathway that allows the bacterium to use cyclopentanol as a sole carbon source for growth. In the present invention there is reported the localization of the CPMO-encoding gene (cpnB) on a 4.3-kb SphI fragment, the determination of its sequence. The 550-amino acid CPMO polypeptide (Mt, 62,111) encoded by the gene was found to have 36.5% identity with the sequence of cyclohexanone 1,2-monooxygenase (CHMO) of Acinetobacter sp. strain NCIMB 9871. The 62-kDa CPMO was expressed in E. coli as an IPTG-inducible protein.
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