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公开(公告)号：US07217559B2
公开(公告)日：2007-05-15
申请号：US10489883
申请日：2002-09-18
申请人： Hiroaki Iwaki , Yoshie Hasegawa , Peter C. K. Lau
发明人： Hiroaki Iwaki , Yoshie Hasegawa , Peter C. K. Lau
CPC分类号： C12N9/0073 , C12N1/20 , C12P17/08 , C12R1/40
摘要： The invention relates to a new strain of Pseudomonas putida (designated as HI-70) and to the isolation, cloning, and sequencing of a cyclododecanone monooxygenase-encoding gene (named cdnB) from said strain. The invention also relates to a new cyclododecanone monooxygenase and to a method of use of the cyclododecanone monooxygenase-encoding gene.
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2.发明授权Cloning, sequencing and expression of a comamonas cyclopentanone 1,2-monooxygenase-encoding gene in Escherichia coli 失效在大肠杆菌中克隆，测序和表达环戊烯酮-1,2-单加氧酶基因公开(公告)号：US07214520B2
公开(公告)日：2007-05-08
申请号：US10312585
申请日：2001-07-13
申请人： Hiroaki Iwaki , Yoshie Hasegawa , Peter C. K. Lau
发明人： Hiroaki Iwaki , Yoshie Hasegawa , Peter C. K. Lau
CPC分类号： C12N9/0073 , C12Y114/13016
摘要： Cyclopentanone 1,2-monooxygenase (CPMO) from Comamonas (previously Pseudomonas) sp. strain NCIMB 9872 carries out the second step of a degradation pathway that allows the bacterium to use cyclopentanol as a sole carbon source for growth. In the present invention there is reported the localization of the CPMO-encoding gene (cpnB) on a 4.3-kb SphI fragment, the determination of its sequence. The 550-amino acid CPMO polypeptide (Mt, 62,111) encoded by the gene was found to have 36.5% identity with the sequence of cyclohexanone 1,2-monooxygenase (CHMO) of Acinetobacter sp. strain NCIMB 9871. The 62-kDa CPMO was expressed in E. coli as an IPTG-inducible protein.
摘要翻译： 来自Comamonas（以前为假单胞菌）的环戊酮1,2-单加氧酶（CPMO） 菌株NCIMB 9872进行降解途径的第二步，其允许细菌使用环戊醇作为生长的唯一碳源。 在本发明中，报道了CPMO编码基因（cpnB）在4.3kb SphI片段上的定位，确定其序列。 发现由该基因编码的550个氨基酸的CPMO多肽（MATT，62,111）与不动杆菌属的环己酮1,2-单加氧酶（CHMO）的序列具有36.5％的同一性。 菌株NCIMB 9871.62-kDa CPMO在大肠杆菌中表达为IPTG诱导蛋白。
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![Recombinant cyclopentanone monooxygenase [cpmo]](/abs-image/US/2009/06/02/US07541168B2/abs.jpg.150x150.jpg)
公开(公告)号：US07541168B2
公开(公告)日：2009-06-02
申请号：US11727730
申请日：2007-03-28
申请人： Hiroaki Iwaki , Yoshie Hasegawa , Peter C. K. Lau
发明人： Hiroaki Iwaki , Yoshie Hasegawa , Peter C. K. Lau
CPC分类号： C12N9/0073 , C12Y114/13016
摘要： Cyclopentanone 1,2-monooxygenase (CPMO) from Comamonas (previously Pseudomonas) sp. strain NCIMB 9872 carries out the second step of a degradation pathway that allows the bacterium to use cyclopentanol as a sole carbon source for growth. In the present invention there is reported the localization of the CPMO-encoding gene (cpnB) on a 4.3-kb SphI fragment, the determination of its sequence. The 550-amino acid CPMO polypeptide (Mr, 62,111) encoded by the gene was found to have 36.5% identity with the sequence of cyclohexanone 1,2-monooxygenase (CHMO) of Acinetobacter sp. strain NCIMB 9871. The 62-kDa CPMO was expressed in E. coli as an IPTG-inducible protein.
摘要翻译： 来自Comamonas（以前为假单胞菌）的环戊酮1,2-单加氧酶（CPMO） 菌株NCIMB 9872进行降解途径的第二步，其允许细菌使用环戊醇作为生长的唯一碳源。 在本发明中，报道了CPMO编码基因（cpnB）在4.3kb SphI片段上的定位，确定其序列。 发现由该基因编码的550个氨基酸的CPMO多肽（Mr，62,111）与不动杆菌属的环己酮1,2-单加氧酶（CHMO）的序列具有36.5％的同一性。 菌株NCIMB 9871.62-kDa CPMO在大肠杆菌中表达为IPTG诱导蛋白。
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公开(公告)号：US07425434B2
公开(公告)日：2008-09-16
申请号：US11717247
申请日：2007-03-13
申请人： Hiroaki Iwaki , Yoshie Hasegawa , Peter C. K. Lau
发明人： Hiroaki Iwaki , Yoshie Hasegawa , Peter C. K. Lau
CPC分类号： C12N9/0073 , C12N1/20 , C12P17/08 , C12R1/40
摘要： The invention relates to a new strain of Pseudomonas putida (designated as HI-70) and to the isolation, cloning, and sequencing of a cyclododecanone monooxygenase-encoding gene (named cdnB) from said strain. The invention also relates to a new cyclododecanone monooxygenase and to a method of use of the cyclododecanone monooxygenase-encoding gene.
摘要翻译： 本发明涉及恶臭假单胞菌（称为HI-70）的新菌株，并且从所述菌株中分离，克隆和测序来自所述菌株的环十二酮单加氧酶编码基因（命名为cdnB）。 本发明还涉及新的环十二酮单加氧酶和使用环十二酮单加氧酶编码基因的方法。
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公开(公告)号：US07745592B2
公开(公告)日：2010-06-29
申请号：US10135362
申请日：2002-05-01
申请人： Bernard Massie , Alaka Mullick , Peter C. K. Lau , Yasuo Konishi
发明人： Bernard Massie , Alaka Mullick , Peter C. K. Lau , Yasuo Konishi
CPC分类号： C07K14/4705 , A01K2217/05 , C07K2319/71 , C12N15/85 , C12N2799/022 , C12N2830/002 , C12N2830/005 , C12N2830/15 , C12N2840/20
摘要： The invention relates to a new “gene-switch” (cumate-inducible switch) for mammalian cells. This switch is as useful in the development of expression systems and cell-based assays for functional genomics as in the generation of viral vectors for gene therapy.
摘要翻译： 本发明涉及用于哺乳动物细胞的新的“基因开关”（cumate-inducible switch）。 该开关对于功能基因组学的表达系统和基于细胞的测定的开发与在基因治疗的病毒载体的产生中是有用的。
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公开(公告)号：US08163894B2
公开(公告)日：2012-04-24
申请号：US12769048
申请日：2010-04-28
申请人： Bernard Massie , Alaka Mullick , Peter C. K. Lau , Yasuo Konishi
发明人： Bernard Massie , Alaka Mullick , Peter C. K. Lau , Yasuo Konishi
CPC分类号： C07K14/4705 , A01K2217/05 , C07K2319/71 , C12N15/85 , C12N2799/022 , C12N2830/002 , C12N2830/005 , C12N2830/15 , C12N2840/20
摘要： The invention relates to a new “gene-switch” (cumate-inducible switch) for mammalian cells, having a mammalian promoter which has a TATA element and is linked to the coding sequence of CymR. This switch is as useful in the development of expression systems and cell-based assays for functional genomics as in the generation of viral vectors for gene therapy.
摘要翻译： 本发明涉及用于哺乳动物细胞的新的“基因开关”（cumate-inducible switch），其具有具有TATA元件并与CymR的编码序列连接的哺乳动物启动子。 该开关对于功能基因组学的表达系统和基于细胞的测定的开发与在基因治疗的病毒载体的产生中是有用的。
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7.发明授权公开(公告)号：US08062877B2
公开(公告)日：2011-11-22
申请号：US12451346
申请日：2008-05-05
摘要： Using site-directed mutagenesis to mutate the Xanthomonas campestris pectate lyase gene, variants of Xanthomonas campestris pectate lyase with improved thermostability and/or enzymatic activity have been expressed in Escherichia coli, and then isolated and purified. The mutant Xanthomonas campestris pectate lyases are more effective than the wild-type enzyme, also expressed in E. coli, in removing pectic compounds from natural hemp fiber.
摘要翻译： 使用定点诱变突变野油菜黄单胞菌果胶裂解酶基因，在大肠杆菌中已经表达了具有改善的热稳定性和/或酶活性的黄单突变果胶裂解酶的变体，然后分离纯化。 突变的野油菜黄单胞菌果胶裂解酶比在大肠杆菌中表达的野生型酶更有效地从天然大麻纤维中除去果胶化合物。
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