The invention concerns a method for estimating the transfer function of a multicarrier signal transmission channel consisting of a temporal succession of symbols of a set of data elements, each of the data elements modulating a carrier frequency of the signal, the data elements comprising pilots and so-called informative data elements. The invention is characterized in that said method comprises: a step which consists in determining a set of two-dimensional discrete prolate spheroidal sequences (DPSS); a step which consists in writing the transfer function in the form of a combination of at least some of the two-dimensional discrete prolate spheroidal sequences (DPSS); a step which consists in time-based and frequency-based two-dimensional interpolation of at least some of the coefficients of the combination, so as to obtain an estimation of the transfer function at any point of a time-frequency network.