发明授权
- 专利标题： Method of selective peptide isolation for the identification and quantitative analysis of proteins in complex mixtures
- 专利标题（中）： 选择性肽分离方法用于鉴定和定量分析复杂混合物中的蛋白质
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申请号： US10988943申请日： 2004-11-15
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公开(公告)号： US07244411B2公开(公告)日： 2007-07-17
- 发明人: Lázaro Hiram Betancourt Núñez , Jorge Fernández de Cossio Dorta-Duque , Vladimir Armando Besada Pérez , Jeovanis Gil Valdés , Luis Javier González López , Gabriel Ramón Padrón Palomares , Rolando Pajón Feyt , Félix Modesto Álvarez Gil
- 申请人： Lázaro Hiram Betancourt Núñez , Jorge Fernández de Cossio Dorta-Duque , Vladimir Armando Besada Pérez , Jeovanis Gil Valdés , Luis Javier González López , Gabriel Ramón Padrón Palomares , Rolando Pajón Feyt , Félix Modesto Álvarez Gil
- 申请人地址： CU C. Habana
- 专利权人： Centro de Ingenieria Genetica y Biotecnologia
- 当前专利权人： Centro de Ingenieria Genetica y Biotecnologia
- 当前专利权人地址： CU C. Habana
- 代理机构： Hoffmann & Baron, LLP
- 优先权： CU1/03 20031119
- 主分类号： A61K51/00
- IPC分类号： A61K51/00 ; A61M36/14
摘要：
The present invention describes a method of selective peptide isolation for the identification and quantitative analysis of proteins in complex mixture. The method comprises the selective isolation from every protein of those peptides that neither contain arginine nor histidine (NHNR peptides), and the determination of the relative concentrations of one or several proteins in different samples from the ratio between the areas of the estimated theoretical spectra for the NHNR peptides labeled with different isotopes in each sample. The determination of the relative concentration of proteins is valid for any type of isotopic label of the NHNR peptides. The method avoids the separation and purification of the proteins present in a complex mixture, and the analysis of all peptides generated from the enzymatic digest of the samples. The method is applicable to the identification of proteins with vacunal, therapeutic and diagnostic aims.
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