发明授权
- 专利标题： Soluble complex comprising a retroviral surface glycoprotein
- 专利标题（中）： 包含逆转录病毒表面糖蛋白的可溶性复合物
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申请号： US11088599申请日： 2005-03-24
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公开(公告)号： US07244575B2公开(公告)日： 2007-07-17
- 发明人: Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Dorothea Sizmann
- 申请人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Dorothea Sizmann
- 申请人地址： US IN Indianapolis
- 专利权人： Roche Diagnostics Corporation
- 当前专利权人： Roche Diagnostics Corporation
- 当前专利权人地址： US IN Indianapolis
- 代理机构： Brinks Hofer Gilson & Lione
- 优先权： EP01115225 20010622; EP01120939 20010831
- 主分类号： C07K14/00
- IPC分类号： C07K14/00 ; G01N33/53
摘要：
The present invention relates to the diagnosis of HIV infections. It especially teaches the production of a soluble retroviral surface glycoprotein-(or transmembrane glycoprotein)-chaperone complex and the advantageous use of a chaperone-antigen complex especially in the detection of antibodies to HIV in immunoassays, preferably according to the double antigen bridge concept, or as an immunogen. The invention also discloses soluble complexes comprising a variant of HIV-1 gp41 or a variant of HIV-2 gp36, respectively, and a chaperone selected from the peptidyl-prolyl-isomerase class of chaperones. Variants comprising specific amino-acid substitutions in the N-helical domain of HIV-1 gp41 or of HIV-2 gp36, respectively, are also described.