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    Soluble rubella E1 envelope protein variants
    7.
    发明授权
    Soluble rubella E1 envelope protein variants 有权
    可溶性风疹E1包膜蛋白变体

    公开(公告)号：US07604935B2

    公开(公告)日：2009-10-20

    申请号：US11584887

    申请日：2006-10-23

    申请人： Barbara Upmeier ,  Ralf Bollhagen ,  Alfred Engel ,  Elke Faatz ,  Peter Schaarschmidt ,  Christian Scholz ,  Toralf Zarnt

    发明人： Barbara Upmeier ,  Ralf Bollhagen ,  Alfred Engel ,  Elke Faatz ,  Peter Schaarschmidt ,  Christian Scholz ,  Toralf Zarnt

    IPC分类号： C12Q1/70 ,  C12N7/01 ,  A61K39/20

    CPC分类号： C07K14/005 ,  C07K2319/35 ,  C12N2770/36222 ,  Y10S530/826

    摘要： A soluble rubella E1 antigen variant is disclosed that comprises amino acids 334-409 of the native rubella E1 peptide, but lacks the C-terminal end and at least the transmembrane region and the anchor segment as well as at least the amino acids 143 to 164. Also described is a recombinant DNA molecule encoding the rubella E1 antigen variants which are recombinantly expressed as a chaperone fusion protein, refolded into a soluble and immunoreactive conformation, and further used for the serological detection of anti-rubella antibodies. In addition, also disclosed is a method for the detection, determination and quantification of anti-rubella antibodies of IgG and/or IgM subclass in a sample wherein the rubella E1 antigen is used as a capture reagent and/or binding partner for the antibodies.

    摘要翻译： 公开了可溶性风疹E1抗原变体，其包含天然风疹E1肽的氨基酸334-409，但缺少C末端和至少跨膜区和锚定区以及至少氨基酸143至164 还描述了编码风疹E1抗原变体的重组DNA分子，其被重组表达为伴侣融合蛋白，重折叠成可溶性和免疫反应性构象，并进一步用于抗风疹抗体的血清学检测。 此外，还公开了用于检测，测定和定量样品中IgG和/或IgM亚类的抗风疹抗体的方法，其中所述风疹E1抗原用作抗体的捕获试剂和/或结合配偶体。

    Soluble rubella E1 envelope protein variants
    8.
    发明申请
    Soluble rubella E1 envelope protein variants 有权
    可溶性风疹E1包膜蛋白变体

    公开(公告)号：US20070154883A1

    公开(公告)日：2007-07-05

    申请号：US11584887

    申请日：2006-10-23

    申请人： Barbara Upmeier ,  Ralf Bollhagen ,  Alfred Engel ,  Elke Faatz ,  Peter Schaarschmidt ,  Christian Scholz ,  Toralf Zarnt

    发明人： Barbara Upmeier ,  Ralf Bollhagen ,  Alfred Engel ,  Elke Faatz ,  Peter Schaarschmidt ,  Christian Scholz ,  Toralf Zarnt

    IPC分类号： C12Q1/70

    CPC分类号： C07K14/005 ,  C07K2319/35 ,  C12N2770/36222 ,  Y10S530/826

    摘要： The invention disclosed relates to a soluble rubella E1 antigen and variants of this peptide characterized by lacking at the C-terminal end at least the transmembrane region and the anchor segment as well as at least the amino acids 143 to 164 and containing at least the region spanning the disulfide bridges Cys 349-Cys 352 and Cys 368-401 whereas the N-terminus (Cys 349) of this region contains additionally at least 15 amino acids and/or the C-terminus (Cys 401) of this region contains additionally at least 8 amino acids of the adjacent rubella E1 antigen sequence. Also described are a recombinant DNA molecule encoding the rubella E1 antigen and variants which are recombinantly expressed as a chaperone fusion protein, refolded into a soluble and immunoreactive conformation, and further used for the serological detection of anti-rubella antibodies. In addition, also disclosed is a method for the detection, determination and quantification of anti-rubella antibodies of IgG and/or IgM subclass in a sample wherein the rubella E1 antigen is used as a capture reagent and/or binding partner for the antibodies.

    摘要翻译： 所公开的本发明涉及可溶性风疹E1抗原和该肽的变体，其特征在于至少在C-末端缺少至少跨膜区和锚段以及至少氨基酸143至164并且至少含有该区域 跨越二硫键Cys 349-Cys 352和Cys 368-401，而该区域的N末端（Cys 349）另外包含至少15个氨基酸和/或该区域的C末端（Cys 401） 相邻风疹E1抗原序列的至少8个氨基酸。 还描述了编码风疹E1抗原的重组DNA分子和重组表达为伴侣融合蛋白的变体，重新折叠成可溶性和免疫反应性构象，并进一步用于抗风疹抗体的血清学检测。 此外，还公开了用于检测，测定和定量样品中IgG和/或IgM亚类的抗风疹抗体的方法，其中所述风疹E1抗原用作抗体的捕获试剂和/或结合配偶体。

    Fusion protein comprising an E. coli chaperone protein and a human chaperone protein
    10.
    发明授权
    Fusion protein comprising an E. coli chaperone protein and a human chaperone protein 有权
    包含大肠杆菌伴侣蛋白和人伴侣蛋白的融合蛋白

    公开(公告)号：US07947494B2

    公开(公告)日：2011-05-24

    申请号：US12166574

    申请日：2008-07-02

    申请人： Christian Scholz ,  Elke Faatz ,  Peter Schaarschmidt ,  Urban Schmitt

    发明人： Christian Scholz ,  Elke Faatz ,  Peter Schaarschmidt ,  Urban Schmitt

    IPC分类号： C12N15/00 ,  C07H21/04

    CPC分类号： C12N9/90 ,  C07K2319/35

    摘要： The invention discloses the cloning, expression and uses of a chimeric fusion protein with superior chaperone and folding activities compared to the wild type chaperones. This invention relates to a chimeric fusion protein encoded by a recombinant DNA molecule comprising nucleotide sequences coding for a polypeptide binding segment of a non-human chaperone protein and nucleotide sequences coding for an FK506 binding protein (FKBP) or an FK506-binding-protein-like domain (FKBP-like domain). In particular, this invention relates to a chimeric fusion protein encoded by a recombinant DNA molecule comprising nucleotide sequences coding for a polypeptide binding segment of a non-human chaperone protein and nucleotide sequences coding for a human FKBP type peptidyl-prolyl-cis/trans isomerase (PPIase), methods of producing these chimeric fusion proteins and their uses as folding helpers in the production of other proteins and in the process of the production of vaccines or pharmaceuticals, and as folding helpers for performing immunoassays.

    摘要翻译： 本发明公开了与野生型伴侣相比，具有优异的伴侣和折叠活性的嵌合融合蛋白的克隆，表达和用途。 本发明涉及由重组DNA分子编码的嵌合融合蛋白，其包含编码非人伴侣蛋白多肽结合片段的核苷酸序列和编码FK506结合蛋白（FKBP）或FK506-结合蛋白 - 像域（FKBP样域）。 特别地，本发明涉及由重组DNA分子编码的嵌合融合蛋白，其包含编码非人伴侣蛋白多肽结合片段的核苷酸序列和编码人FKBP型肽基 - 脯氨酰 - 顺/反异构酶的核苷酸序列 （PPIase），生产这些嵌合融合蛋白的方法及其在生产其他蛋白质和疫苗或药物生产过程中作为折叠助剂的用途，以及用于进行免疫测定的折叠助剂。