发明授权
- 专利标题： Protein and processes for producing the same
- 专利标题（中）： 蛋白质及其制备方法
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申请号： US09647907申请日： 1999-04-09
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公开(公告)号： US07252973B1公开(公告)日： 2007-08-07
- 发明人: Masaaki Goto , Akihiro Tomoyasu , Kazuki Yano , Fumie Kobayashi , Nobuaki Nakagawa , Hisataka Yasuda , Kyoji Yamaguchi , Masahiko Kinosaki , Shin-ichi Mochizuki , Tadashi Nakakarumai , Tomonori Morinaga , Eisuke Tsuda , Kanji Higashio
- 申请人： Masaaki Goto , Akihiro Tomoyasu , Kazuki Yano , Fumie Kobayashi , Nobuaki Nakagawa , Hisataka Yasuda , Kyoji Yamaguchi , Masahiko Kinosaki , Shin-ichi Mochizuki , Tadashi Nakakarumai , Tomonori Morinaga , Eisuke Tsuda , Kanji Higashio
- 申请人地址： JP Tokyo
- 专利权人： Daiichi Pharmaceutical Co., Ltd.
- 当前专利权人： Daiichi Pharmaceutical Co., Ltd.
- 当前专利权人地址： JP Tokyo
- 代理机构： Oblon, Spivak, McClelland, Maier & Neustadt, P.C.
- 优先权： JP10-099741 19980410; JP10-243355 19980828
- 国际申请： PCT/JP99/01906 WO 19990409
- 国际公布： WO99/53056 WO 19991021
- 主分类号： C12P21/04
- IPC分类号： C12P21/04 ; C12N5/02 ; C07K1/00
摘要：
A novel protein and a process of producing the protein is provided. The protein is a glycoprotein having activity of suppressing the differentiation and/or maturation of adipocyte, having a molecular weight of about 45 kD under non-reducing conditions and about 28 kD and/or 23 kD under reducing conditions, and exhibiting affinity to heparin. A process of producing the protein comprising culturing human fibroblasts and purifying the culture broth by chromatography using an ion exchange column, affinity column, and reverse phase column.A cDNA encoding the protein and a process of producing the protein using the cDNA are also provided. The protein of the present invention is useful as a pharmaceutical composition for preventing or treating obesity or as an antigen for establishing immunological diagnosis, etc.
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