发明授权
US07270972B1 Process for producing subunit peptide originating in polymer protein
失效
用于产生源自聚合物蛋白质的亚单位肽的方法
- 专利标题： Process for producing subunit peptide originating in polymer protein
- 专利标题（中）： 用于产生源自聚合物蛋白质的亚单位肽的方法
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申请号： US09926256申请日： 2000-03-31
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公开(公告)号： US07270972B1公开(公告)日： 2007-09-18
- 发明人: Naoyuki Fukuchi , Shunsuke Kageyama , Morikazu Kito , Takashi Kayahara , Hiroshi Yamamoto
- 申请人： Naoyuki Fukuchi , Shunsuke Kageyama , Morikazu Kito , Takashi Kayahara , Hiroshi Yamamoto
- 申请人地址： JP Tokyo
- 专利权人： Ajinomoto Co., Inc.
- 当前专利权人： Ajinomoto Co., Inc.
- 当前专利权人地址： JP Tokyo
- 代理机构： Oblon, Spivak, McClelland, Maier & Neustadt, P.C.
- 优先权： JP11/096073 19990402
- 国际申请： PCT/JP00/02127 WO 20000331
- 国际公布： WO00/59926 WO 20001012
- 主分类号： C12Q1/56
- IPC分类号： C12Q1/56 ; A61K38/00
摘要：
It is provided a method for producing a subunit peptide originating from an oligomeric protein having disulfide bonds within a subunit and between subunits. The method comprises producing a subunit originating from an oligomeric protein having disulfide bonds within a subunit and between subunits by the following steps: (a) a step of refolding the subunit by denaturing the oligomeric protein or its subunit in a solution with a protein-denaturing agent and removing the denaturing agent from the solution in the presence of polyoxyalkyl polyether having a functional group that reacts with a thiol group; and (b) a step of isolating the subunit bonded to the polyoxyalkyl polyether from the solution.