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发明授权
US07332322B2 Venezuelan equine encephalitis virus replicons with adaptive mutations in the genome and uses thereof 有权
委内瑞兰马脑炎病毒复制子与基因组中的适应性突变及其用途

  • 专利标题： Venezuelan equine encephalitis virus replicons with adaptive mutations in the genome and uses thereof
  • 专利标题（中）： 委内瑞兰马脑炎病毒复制子与基因组中的适应性突变及其用途
  • 申请号： US11227671
    申请日： 2005-09-14
  • 公开(公告)号： US07332322B2
    公开(公告)日： 2008-02-19
  • 发明人: Ilya Frolov , Elena Frolova
  • 申请人： Ilya Frolov , Elena Frolova
  • 代理商 Benjamin Aaron Adler
  • 主分类号： C12N7/04
  • IPC分类号： C12N7/04 ;  C12N15/86 ;  C12P21/04 ;  C12P19/34 ;  C12Q1/68
Venezuelan equine encephalitis virus replicons with adaptive mutations in the genome and uses thereof
摘要：
The present invention provides a Venezuelan equine encephalitis virus replicon RNA useful in the development of stable lines of mammalian, avian and insect cells in which these replicons will persistently replicate. Venezuelan equine encephalitis (VEE) virus replicons contain a number of unique adaptive mutations that make the replicons noncytopathic. The replicons remain resistant to IFN-α/β. Replicon replication leads to high-level production of heterologous proteins, which are encoded by the replicons' genome and are under the control of a viral subgenomic promoter. Also provided are methods of screening for inhibitory compounds of Venezuelan equine encephalitis virus replication and eastern equine encephalitis virus replication.
摘要（中）：

本发明提供了一种可用于发展哺乳动物，禽和昆虫细胞的稳定系的委内瑞兰马脑炎病毒复制子RNA，其中这些复制子将持续复制。 委内瑞兰马脑炎（VEE）病毒复制子包含许多独特的适应性突变，使复制子非细胞病变。 复制子对IFN-α/β仍然具有抗性。 复制子复制导致异源蛋白质的高水平生产，其由复制子基因组编码并在病毒亚基因组启动子的控制下。 还提供了筛选委内瑞兰马脑炎病毒复制和东马脑炎病毒复制的抑制性化合物的方法。

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