In a sheet-fed scanner capable of scanning multiple scan lines as a calibration standard for an image signal, a scanning module scans a document, which is fed across a scan region by a sheet-feeding mechanism, and a stationary calibration sheet in the scan region. The scanning module includes an image sensor, a lens and at least one reflecting mirror. An actuator actuates the reflecting mirror to enable the image sensor to sense a plurality of scan lines on the calibration sheet through the lens and the reflecting mirror in order to obtain a calibration standard for the image signal of the document. The calibration standard is adopted to calibrate an image signal of the document, and with this invention, it is possible to avert any flaws in image quality caused by the contaminated calibration sheet.