发明授权
US07399607B2 Fluorescence polarization assays for determining clostridial toxin activity
有权
用于测定梭菌毒素活性的荧光偏振测定法
- 专利标题： Fluorescence polarization assays for determining clostridial toxin activity
- 专利标题（中）： 用于测定梭菌毒素活性的荧光偏振测定法
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申请号： US10948097申请日： 2004-09-22
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公开(公告)号： US07399607B2公开(公告)日： 2008-07-15
- 发明人: Dudley J. Williams , Marcella Gilmore , Lance Steward , Marc Verhagen , Kei Roger Aoki
- 申请人： Dudley J. Williams , Marcella Gilmore , Lance Steward , Marc Verhagen , Kei Roger Aoki
- 申请人地址： US CA Irvine
- 专利权人： Allergan, Inc.
- 当前专利权人： Allergan, Inc.
- 当前专利权人地址： US CA Irvine
- 代理商 Dean G. Stathakis; Joel B. German; Martin A. Voet
- 主分类号： C12Q1/37
- IPC分类号： C12Q1/37 ; C12N11/16
摘要：
The present invention provides a method of determining the presence or activity of a clostridial toxin by (a) treating with a sample, under conditions suitable for clostridial toxin protease activity, a clostridial toxin substrate which includes a fluorophore; a bulking group; and a clostridial toxin recognition sequence containing a cleavage site that intervenes between the fluorophore and the bulking group; (b) exciting the fluorophore with plane polarized light; and (c) determining fluorescence polarization of the treated substrate relative to a control substrate, where a change in fluorescence polarization of the treated substrate as compared to fluorescence polarization of the control substrate is indicative of the presence or activity of the clostridial toxin.
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