发明授权
- 专利标题: Fluorescence polarization instruments and methods for detection of exposure to biological materials by fluorescence polarization immunoassay of saliva, oral or bodily fluids
- 专利标题(中): 荧光偏振仪器和通过唾液,口腔或体液荧光偏振免疫测定检测生物材料的方法
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申请号: US10700868申请日: 2003-11-05
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公开(公告)号: US07408640B2公开(公告)日: 2008-08-05
- 发明人: Malford E. Cullum , Lloyd G. Simonson , Sylvia Z. Schade , Linda A. Lininger , Alan L. McArthur
- 申请人: Malford E. Cullum , Lloyd G. Simonson , Sylvia Z. Schade , Linda A. Lininger , Alan L. McArthur
- 申请人地址: US DC Washington
- 专利权人: The United States of America as represented by the Secretary of the Navy
- 当前专利权人: The United States of America as represented by the Secretary of the Navy
- 当前专利权人地址: US DC Washington
- 代理商 Ning Yang; Joseph K. Hemby
- 主分类号: G01J3/00
- IPC分类号: G01J3/00 ; G01J4/00 ; C12M1/00 ; C12M3/00
摘要:
The inventive subject matter relates to a method for detecting the presence of a biological substance of interest in a test sample of saliva or oral fluid, comprising combining said test sample with a fluorescence-labeled ligand to said biological substance and detecting a change in the fluorescence polarization of said test sample produced by binding of said fluorescence-labeled ligand to said biological substance. In one aspect of the inventive subject matter, said method comprises additional steps for comparing the fluorescence polarization of said test sample with the fluorescence polarization of a control solution. Also provided is a miniaturized, portable apparatus for measuring the fluorescence polarization of a liquid sample.
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