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1.发明授权公开(公告)号：US08114582B2
公开(公告)日：2012-02-14
申请号：US12163412
申请日：2008-06-27
申请人： Malford E. Cullum , Lloyd G. Simonson , Sylvia Z. Schade , Linda A. Lininger , Alan L. McArthur
发明人： Malford E. Cullum , Lloyd G. Simonson , Sylvia Z. Schade , Linda A. Lininger , Alan L. McArthur
CPC分类号： G01N21/6428 , G01N21/6445 , G01N33/582 , G01N2201/0221 , Y10T436/143333
摘要： The inventive subject matter relates to a method for detecting the presence of a biological substance of interest in a test sample of saliva or oral fluid, comprising combining said test sample with a fluorescence-labeled ligand to said biological substance and detecting a change in the fluorescence polarization of said test sample produced by binding of said fluorescence-labeled ligand to said biological substance. In one aspect of the inventive subject matter, said method comprises additional steps for comparing the fluorescence polarization of said test sample with the fluorescence polarization of a control solution. Also provided is a miniaturized, portable apparatus for measuring the fluorescence polarization of a liquid sample.
摘要翻译： 本发明涉及一种用于检测唾液或口腔液测试样品中感兴趣的生物物质的存在的方法，包括将所述测试样品与荧光标记的配体结合到所述生物物质上并检测荧光的变化 通过将所述荧光标记的配体结合到所述生物物质而产生的所述测试样品的极化。 在本发明主题的一个方面，所述方法包括用于将所述测试样品的荧光偏振与对照溶液的荧光偏振进行比较的附加步骤。 还提供了用于测量液体样品的荧光偏振的小型便携式设备。
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2.发明申请FLUORESCENCE POLARIZATION INSTRUMENTS AND METHODS FOR DETECTION OF EXPOSURE TO BIOLOGICAL MATERIALS BY FLUORESCENCE POLARIZATION IMMUNOASSAY OF SALIVA, ORAL OR BODILY FLUIDS 有权荧光偏振仪和通过荧光偏振检测生物材料暴露的方法，SALIVA，口腔或体液的免疫荧光公开(公告)号：US20080318266A1
公开(公告)日：2008-12-25
申请号：US12163412
申请日：2008-06-27
申请人： Malford E. Cullum , Lloyd G. Simonson , Sylvia Z. Schade , Linda A. Lininger , Alan L. McArthur
发明人： Malford E. Cullum , Lloyd G. Simonson , Sylvia Z. Schade , Linda A. Lininger , Alan L. McArthur
CPC分类号： G01N21/6428 , G01N21/6445 , G01N33/582 , G01N2201/0221 , Y10T436/143333
摘要： The inventive subject matter relates to a method for detecting the presence of a biological substance of interest in a test sample of saliva or oral fluid, comprising combining said test sample with a fluorescence-labeled ligand to said biological substance and detecting a change in the fluorescence polarization of said test sample produced by binding of said fluorescence-labeled ligand to said biological substance. In one aspect of the inventive subject matter, said method comprises additional steps for comparing the fluorescence polarization of said test sample with the fluorescence polarization of a control solution. Also provided is a miniaturized, portable apparatus for measuring the fluorescence polarization of a liquid sample.
摘要翻译： 本发明涉及一种用于检测唾液或口腔液测试样品中感兴趣生物物质的存在的方法，包括将所述测试样品与荧光标记的配体结合到所述生物物质上并检测荧光的变化 通过将所述荧光标记的配体结合到所述生物物质而产生的所述测试样品的极化。 在本发明主题的一个方面，所述方法包括用于将所述测试样品的荧光偏振与对照溶液的荧光偏振进行比较的附加步骤。 还提供了用于测量液体样品的荧光偏振的小型便携式设备。
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3.发明授权Rapid immunoassay of anthrax protective antigen in vaccine cultures and bodily fluids by fluorescence polarization 失效通过荧光偏振在疫苗培养物和体液中快速免疫测定炭疽保护性抗原公开(公告)号：US07504202B2
公开(公告)日：2009-03-17
申请号：US10809877
申请日：2004-03-26
申请人： Malford E. Cullum , Paul Hine , Lloyd G. Simonson , Chun N. Shih , Diane R. Bienek , Sukjoon Park , James C. Ragain, Jr. , Linda A. Lininger
发明人： Malford E. Cullum , Paul Hine , Lloyd G. Simonson , Chun N. Shih , Diane R. Bienek , Sukjoon Park , James C. Ragain, Jr. , Linda A. Lininger
IPC分类号： C12Q1/00
CPC分类号： G01N33/56911
摘要： The inventive subject matter relates to a competitive method for estimating the concentration in a sample of a Bacillus anthracis protein or antibody thereof selected from the group consisting of protective antigen (PA), lethal factor (LF) and edema factor (EF). The method may employ the use of Fluorescence Polarization, FLT or FRET. The competitive methods are capable of detecting a target protein within 5 minutes within the range of 0.1 to 10.0 nM. The methods provide for the rapid detection and quantitation of bacteria, bacterial antigen or antibody in culture media or broth of growing cultures of bacteria, including B. anthracis by fluorescent methods.
摘要翻译： 本发明涉及用于估计选自保护性抗原（PA），致死因子（LF）和水肿因子（EF）的炭疽杆菌蛋白质或其抗体样品中的浓度的竞争方法。 该方法可以使用荧光偏振，FLT或FRET。 竞争性方法能够在0.1至10.0nM范围内的5分钟内检测靶蛋白。 该方法提供了通过荧光方法在细菌生长培养物（包括炭疽杆菌）的培养基或肉汤中快速检测和定量细菌，细菌抗原或抗体。
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4.发明授权公开(公告)号：US07408640B2
公开(公告)日：2008-08-05
申请号：US10700868
申请日：2003-11-05
申请人： Malford E. Cullum , Lloyd G. Simonson , Sylvia Z. Schade , Linda A. Lininger , Alan L. McArthur
发明人： Malford E. Cullum , Lloyd G. Simonson , Sylvia Z. Schade , Linda A. Lininger , Alan L. McArthur
CPC分类号： G01N21/6428 , G01N21/6445 , G01N33/582 , G01N2201/0221 , Y10T436/143333
摘要： The inventive subject matter relates to a method for detecting the presence of a biological substance of interest in a test sample of saliva or oral fluid, comprising combining said test sample with a fluorescence-labeled ligand to said biological substance and detecting a change in the fluorescence polarization of said test sample produced by binding of said fluorescence-labeled ligand to said biological substance. In one aspect of the inventive subject matter, said method comprises additional steps for comparing the fluorescence polarization of said test sample with the fluorescence polarization of a control solution. Also provided is a miniaturized, portable apparatus for measuring the fluorescence polarization of a liquid sample.
摘要翻译： 本发明涉及一种用于检测唾液或口腔液测试样品中感兴趣的生物物质的存在的方法，包括将所述测试样品与荧光标记的配体结合到所述生物物质上并检测荧光的变化 通过将所述荧光标记的配体结合到所述生物物质而产生的所述测试样品的极化。 在本发明主题的一个方面，所述方法包括用于将所述测试样品的荧光偏振与对照溶液的荧光偏振进行比较的附加步骤。 还提供了用于测量液体样品的荧光偏振的小型便携式设备。
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