Invention Grant
- Patent Title: Thermostable enzyme promoting the fidelity of thermostable DNA polymerases—for improvement of nucleic acid synthesis and amplification in vitro
- Patent Title (中): 促进耐热DNA聚合酶保真度的热稳定酶 - 用于体外改进核酸合成和扩增
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Application No.: US11241116Application Date: 2005-09-29
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Publication No.: US07410782B2Publication Date: 2008-08-12
- Inventor: Waltraud Ankenbauer , Frank Laue , Harald Sobek , Michael Greif
- Applicant: Waltraud Ankenbauer , Frank Laue , Harald Sobek , Michael Greif
- Applicant Address: DE Mannheim US CA Alameda
- Assignee: Roche Diagnostics GmbH,Roche Molecular Systems, Inc.
- Current Assignee: Roche Diagnostics GmbH,Roche Molecular Systems, Inc.
- Current Assignee Address: DE Mannheim US CA Alameda
- Agency: Townsend and Townsend and Crew LLP
- Priority: EP99119268 19990928
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12Q1/68 ; C07K1/00 ; C07H21/04

Abstract:
A purified thermostable enzyme is derived form the thermophilic archaebacterium Archaeoglobus fulgidus. The enzyme can be native or recombinant, is stable under PCR conditions and exhibits double strand specific exonuclease activity. It is a 3′-5′ exonuclease and cleaves to produce 5′-mononucleotides. Thermostable exonucleases are useful in many recombinant DNA techniques, in combination with a thermostable DNA polymerase like Taq especially for nucleic acid amplification by the polymerase chain reaction (PCR).
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