发明授权
- 专利标题: Restricted glucose feed for animal cell culture
- 专利标题(中): 限制性葡萄糖饲料用于动物细胞培养
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申请号: US10847729申请日: 2004-05-17
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公开(公告)号: US07429491B2公开(公告)日: 2008-09-30
- 发明人: Yen-Tung Luan , Terry Cardoza Stanek , Denis Drapeau
- 申请人: Yen-Tung Luan , Terry Cardoza Stanek , Denis Drapeau
- 申请人地址: US NJ Madison
- 专利权人: Wyeth
- 当前专利权人: Wyeth
- 当前专利权人地址: US NJ Madison
- 代理机构: Fitzpatrick, Cella, Harper & Scinto
- 主分类号: C12N5/02
- IPC分类号: C12N5/02
摘要:
Methods of improving protein production in animal cell cultures are provided. Cell culture methods are presented wherein glucose is fed in a restricted manner to cell culture; this restricted feeding of glucose to the cell culture results in lactate production being controlled to a low level. The restricted feeding of glucose in a fed-batch process is not accomplished through a constant-rate feeding of glucose, and the restricted feeding need not depend on sampling. Instead, restricted feeding of glucose to the culture is accomplished through feeding of glucose to the culture at a rate that is a function of an expected or a premodeled rate of glucose consumption by the animal cells when exposed to medium containing a high level of glucose. Because lactate production is controlled to low levels, recombinant protein production is increased.
公开/授权文献
- US20050070013A1 Restricted glucose feed for animal cell culture 公开/授权日:2005-03-31