发明授权
- 专利标题: Polynucleotides encoding recombinant respiratory syncytial viruses expressing immune modulatory molecules
- 专利标题(中): 编码表达免疫调节分子的重组呼吸道合胞病毒的多核苷酸
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申请号: US10754895申请日: 2004-01-08
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公开(公告)号: US07465794B2公开(公告)日: 2008-12-16
- 发明人: Peter L. Collins , Alexander Bukreyev , Brian R. Murphy , Stephen S. Whitehead
- 申请人: Peter L. Collins , Alexander Bukreyev , Brian R. Murphy , Stephen S. Whitehead
- 申请人地址: US DC Washington
- 专利权人: The United States of America as represented by the Department of Health and Human Services
- 当前专利权人: The United States of America as represented by the Department of Health and Human Services
- 当前专利权人地址: US DC Washington
- 代理机构: Birch, Stewart, Kolasch & Birch, LLP
- 主分类号: C07H21/04
- IPC分类号: C07H21/04 ; C12P21/00 ; A61K39/12 ; A61K39/155
摘要:
Recombinant respiratory syncytial virus (RSV) are provided which express one or more immune modulatory molecules. The recombinant virus is modified by addition or substitution of a polynucleotide sequence encoding the immune modulatory molecule, which is preferably a cytokine. Introduction of the cytokine increase, decrease, or otherwise enhances aspects of viral biology and/or host immune responses to RSV to facilitate vaccine use of the virus. Cytokines for use within the invention include but are not limited to interleukin 2 (IL-2), interleukin 4 (IL-4), interleukin 5 (IL-5), interleukin 6 (IL6), or interleukin 18 (IL-18), tumor necrosis factor (TNF) alpha, interferon gamma (IFN), and granulocyte-macrophage colony stimulating factor (GM-CSF). The polynucleotide or immune modulatory molecule is preferably added or substituted into the recombinant viral genome or antigenome, typically at an intergenic or other non-coding site, as a separate gene but may be otherwise expressed, for example as a fusion protein.
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