发明授权
- 专利标题: Compositions and methods for enhanced expression of immunoglobulins from a single vector using a peptide cleavage site
- 专利标题(中): 使用肽切割位点从单个载体增强免疫球蛋白表达的组合物和方法
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申请号: US10831302申请日: 2004-04-26
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公开(公告)号: US07498024B2公开(公告)日: 2009-03-03
- 发明人: Jianmin Fang , Karin Jooss , Jing-Jing Qian
- 申请人: Jianmin Fang , Karin Jooss , Jing-Jing Qian
- 申请人地址: US CA South San Francisco
- 专利权人: Cell Genesys, Inc.
- 当前专利权人: Cell Genesys, Inc.
- 当前专利权人地址: US CA South San Francisco
- 代理机构: Ropes & Gray LLP
- 代理商 James F. Haley, Jr.; Teresa Chen
- 主分类号: C12N7/01
- IPC分类号: C12N7/01 ; C12N15/00 ; C12N15/11 ; C12N15/13 ; C12N5/10 ; C12P21/00 ; C12P21/08
摘要:
Single vector constructs for expression of an immunoglobulin molecule or fragment thereof and methods of making and using the same are described. The vectors comprise a self-processing cleavage sequence between a first and second immunoglobulin coding sequence allowing for expression of a functional antibody molecule using a single promoter. The vector constructs include the coding sequence for a self-processing cleavage site and may further include an additional proteolytic cleavage sequence which provides a means to remove the self processing peptide sequence from an expressed immunoglobulin molecule or fragment thereof. The vector constructs find utility in methods for enhanced production of biologically active immunoglobulins or fragments thereof in vitro or in vivo.