发明授权
- 专利标题： Method for synthesizing DNA
- 专利标题（中）： DNA合成方法
-
申请号： US09673884申请日： 1999-04-21
-
公开(公告)号： US07521178B1公开(公告)日： 2009-04-21
- 发明人: Kiyozo Asada , Takashi Uemori , Yoshimi Sato , Tomoko Fujita , Kazue Miyake , Osamu Takeda , Hiroyuki Mukai , Ikunoshin Kato
- 申请人： Kiyozo Asada , Takashi Uemori , Yoshimi Sato , Tomoko Fujita , Kazue Miyake , Osamu Takeda , Hiroyuki Mukai , Ikunoshin Kato
- 申请人地址： JP Otsu-shi
- 专利权人： Takara Bio Inc.
- 当前专利权人： Takara Bio Inc.
- 当前专利权人地址： JP Otsu-shi
- 代理机构： Birch, Stewart, Kolasch & Birch, LLP
- 优先权： JP10-114005 19980419; JP10-315243 19981106
- 国际申请： PCT/JP99/02121 WO 19990421
- 国际公布： WO99/54455 WO 19991028
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12P19/34 ; C07H21/02
摘要：
A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase. According to the present invention, DNA synthesis can be carried out at an efficiency more excellent as compared to conventional DNA synthesis reaction.
信息查询
