Invention Grant
US07536263B2 Robust genotyping method using DNA chip having discriminating probe and amplicon probe immobilized thereon and DNA chip used therein 失效
使用固定有鉴别探针和扩增子探针的DNA芯片的稳健基因分型方法和其中使用的DNA芯片

Robust genotyping method using DNA chip having discriminating probe and amplicon probe immobilized thereon and DNA chip used therein
Abstract:
Provided is a genotyping method. The genotyping method includes using a DNA chip on which just an optimal probe set is immobilized, the optimal probe sets composed of a discriminating probe which perfectly matches a mutant type gene or a wild type gene for each of at least one identified mutation site in each of at least one amplicon contained in a sample and an amplicon probe having a sequence which perfectly matches a region excluding a nucleotide sequence at a mutation site in each amplicon and is absent from the other amplicons, in order to identify which one of a wild type gene and a mutant type gene is present in each mutation site. In this genotyping method, the number of probes used can be reduced and reliability of genotyping results can be increased.
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