Invention Grant
US07536263B2 Robust genotyping method using DNA chip having discriminating probe and amplicon probe immobilized thereon and DNA chip used therein
失效
使用固定有鉴别探针和扩增子探针的DNA芯片的稳健基因分型方法和其中使用的DNA芯片
- Patent Title: Robust genotyping method using DNA chip having discriminating probe and amplicon probe immobilized thereon and DNA chip used therein
- Patent Title (中): 使用固定有鉴别探针和扩增子探针的DNA芯片的稳健基因分型方法和其中使用的DNA芯片
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Application No.: US11019011Application Date: 2004-12-21
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Publication No.: US07536263B2Publication Date: 2009-05-19
- Inventor: Hyeong-sop Shim , Jong-min Kim , Ji-young Oh
- Applicant: Hyeong-sop Shim , Jong-min Kim , Ji-young Oh
- Applicant Address: KR
- Assignee: Samsung Electronics Co., Ltd.
- Current Assignee: Samsung Electronics Co., Ltd.
- Current Assignee Address: KR
- Agency: Cantor Colburn LLP
- Priority: KR10-2003-0097806 20031226
- Main IPC: G06F19/00
- IPC: G06F19/00 ; C12Q1/68

Abstract:
Provided is a genotyping method. The genotyping method includes using a DNA chip on which just an optimal probe set is immobilized, the optimal probe sets composed of a discriminating probe which perfectly matches a mutant type gene or a wild type gene for each of at least one identified mutation site in each of at least one amplicon contained in a sample and an amplicon probe having a sequence which perfectly matches a region excluding a nucleotide sequence at a mutation site in each amplicon and is absent from the other amplicons, in order to identify which one of a wild type gene and a mutant type gene is present in each mutation site. In this genotyping method, the number of probes used can be reduced and reliability of genotyping results can be increased.
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