A high-speed PCI interface system with reset function and a reset method thereof are provided. The interface system comprises a host controller chipset, at least one high-speed PCI device and at least one reset signal generator. While a hot reset packet cannot be executed by the high-speed PCI device, the host controller chipset can respectively transmit a trigger signal and a PCI reset signal to each corresponding reset signal generator through a trigger signal line and a PCI reset signal line, and further the reset signal generator operates to generate a basic resetting signal. Finally, the basic resetting signal will be transmitted to the corresponding high-speed PCI device through a basic reset signal line such that the system can be used to operate the basic resetting action without restarting power.