A polarized neutron guide for separating neutrons into polarized neutrons while minimizing loss of the neutrons is provided. The polarized neutron guide includes a body, the first space and the second space, and a neutron separation space. The body includes super mirrors coated with a neutron-reflective thin film and the first and second spaces are formed by the first plate inside the body. The neutron separation space is formed by the second plate disposed at the entry of the first space and the third plate disposed at the entry of the second space. Spin-up polarized neutrons and spin-down polarized neutrons are simultaneously separated and transferred in the first and second spaces, respectively. Therefore, with minimum loss of the neutrons, the spin-up polarized neutrons and the spin-down polarized neutrons are effectively separated and collected.